Protein Info for BPHYT_RS15085 in Burkholderia phytofirmans PsJN

Annotation: aldehyde oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02965: xanthine dehydrogenase, molybdopterin binding subunit
  • amino acids 23 to 782 (760 residues), 1254.3 bits, E=0
• PF01315: Ald_Xan_dh_C
  • amino acids 36 to 136 (101 residues), 93.8 bits, E=1.2e-30
• PF02738: MoCoBD_1
  • amino acids 164 to 399 (236 residues), 268.9 bits, E=5e-84
• PF20256: MoCoBD_2
  • amino acids 426 to 710 (285 residues), 331.5 bits, E=7.6e-103

Best Hits

KEGG orthology group: K13482, xanthine dehydrogenase large subunit [EC: 1.17.1.4] (inferred from 100% identity to bpy:Bphyt_3042)

Predicted SEED Role

"Xanthine dehydrogenase, molybdenum binding subunit (EC 1.17.1.4)" in subsystem Purine Utilization (EC 1.17.1.4)

MetaCyc Pathways

• purine nucleotides degradation I (plants) (10/12 steps found)
• adenosine nucleotides degradation I (7/8 steps found)
• guanosine nucleotides degradation II (4/4 steps found)
• superpathway of purines degradation in plants (14/18 steps found)
• superpathway of guanosine nucleotides degradation (plants) (5/6 steps found)
• adenosine nucleotides degradation II (4/5 steps found)
• guanosine nucleotides degradation I (3/4 steps found)
• guanosine nucleotides degradation III (3/4 steps found)
• inosine 5'-phosphate degradation (3/4 steps found)
• purine nucleotides degradation II (aerobic) (8/11 steps found)
• ureide biosynthesis (5/7 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• caffeine degradation III (bacteria, via demethylation) (1/7 steps found)
• theophylline degradation (1/9 steps found)
• caffeine degradation IV (bacteria, via demethylation and oxidation) (1/10 steps found)

KEGG Metabolic Maps

• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.17.1.4

Use Curated BLAST to search for 1.17.1.4

Sequence Analysis Tools

See B2T670 at UniProt or InterPro

Protein Sequence (800 amino acids)

>BPHYT_RS15085 aldehyde oxidase (Burkholderia phytofirmans PsJN)
MNRTDAFVEHAGHVAHESGAAIGVSLPHESAALHVSGEATYTDDVPELQGTLHAALGLSR
HAHARIVSMDLDAVRKAPGVIAVLTAEDIPGENNCGPVLHDDPILAVNEVLYLGQPVFAV
IAESHELARRAAALAKSDDVIRYEPLEAILTPADAKAAKQFVLPPLHLKRGEPDAKIATA
PNRISGTFEVGGQEQFYLEGQIAYAVPKEMDGMLVYSSTQHPSEMQQVVAHMLDWPQHNV
LCECRRMGGGFGGKESQSAVFACVAALAAKLLRRPVKLRADRDDDFMITGKRHDAVYVYE
AGFDDSGRILGARVEIALRAGYSADLSGAVATRAVCHFDNAYYLSDVDIVALCCKTNTQS
NTAFRGFGGPQGALVMEVMLDSIARQLNCDPLEVRLANYYGIGERDTTPYGQRVEDNIIA
PLTDELLATSGYRARREAIAAFNAGSPVLKRGIAFSPVKFGISFNVPFLNQAGALVHVYK
DGSVLVNHGGTEMGQGLNTKVAQVVANEFGLPLSRVRVTATDTSKIANTSATAASTGSDL
NGKAAEAAAKTIRARLAELVAKQLGGTANDVQFANGEVSVNGGAMPFEQLVGAAYLARVQ
LWSDGFYSTPKVHWDAKTLTGHPFYYFAYGAAVSEVVIDTLTGEWKLVRADVLHDAGQSI
NPAIDIGQVEGGFIQGMGWLTTEELWWNRDGRLMTHAPSTYKIPAVSDTPAAFHVQLYQN
RNAEPTVFRSKAVGEPPLLLPFSVFLAIRDAIAAAVPDAQEAPQLRAPATPEAILDALDA
LQPQPAPQPATAPATNDTTA