Protein Info for BPHYT_RS15080 in Burkholderia phytofirmans PsJN

Annotation: FAD-binding molybdopterin dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02963: xanthine dehydrogenase, small subunit
  • amino acids 34 to 513 (480 residues), 690.3 bits, E=5.9e-212
• PF01799: Fer2_2
  • amino acids 114 to 191 (78 residues), 107.1 bits, E=7.7e-35
• PF00941: FAD_binding_5
  • amino acids 240 to 404 (165 residues), 175.2 bits, E=2.1e-55
• PF03450: CO_deh_flav_C
  • amino acids 411 to 512 (102 residues), 117.3 bits, E=6.1e-38

Best Hits

KEGG orthology group: K13481, xanthine dehydrogenase small subunit [EC: 1.17.1.4] (inferred from 100% identity to bpy:Bphyt_3041)

Predicted SEED Role

"Xanthine dehydrogenase, iron-sulfur cluster and FAD-binding subunit A (1.17.1.4)" in subsystem Purine Utilization

MetaCyc Pathways

• purine nucleotides degradation I (plants) (10/12 steps found)
• adenosine nucleotides degradation I (7/8 steps found)
• guanosine nucleotides degradation II (4/4 steps found)
• superpathway of purines degradation in plants (14/18 steps found)
• superpathway of guanosine nucleotides degradation (plants) (5/6 steps found)
• adenosine nucleotides degradation II (4/5 steps found)
• guanosine nucleotides degradation I (3/4 steps found)
• guanosine nucleotides degradation III (3/4 steps found)
• inosine 5'-phosphate degradation (3/4 steps found)
• purine nucleotides degradation II (aerobic) (8/11 steps found)
• ureide biosynthesis (5/7 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• caffeine degradation III (bacteria, via demethylation) (1/7 steps found)
• theophylline degradation (1/9 steps found)
• caffeine degradation IV (bacteria, via demethylation and oxidation) (1/10 steps found)

KEGG Metabolic Maps

• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.17.1.4

Use Curated BLAST to search for 1.17.1.4

Sequence Analysis Tools

See B2T669 at UniProt or InterPro

Protein Sequence (543 amino acids)

>BPHYT_RS15080 FAD-binding molybdopterin dehydrogenase (Burkholderia phytofirmans PsJN)
MAIYVREAGFGATPCVTRARSAVSSGFAMTTQTIRFYHQGAVREVAGVPASRTVLQHLRE
DLHCTGTKEGCAEGDCGACTVVVGELDAHGGLALKAVNACIQFLPTLDGKALFTVEDLRA
ADGALHPVQQAMVDCHGSQCGFCTPGFVMSMWALYENQPAAAGLPTRDEINAALSGNLCR
CTGYRPIVDASQKMFDYPQYPRVTLDRKAVADQLRELQRRDSFEYRAPDTRGAAFGSPAF
HAPVSLDAFAKLRADHPQARLLAGSTDVGLWVTKQFRDLGDILYIGNVAELKTIERDAQT
LTIGAAVTLEDAYAALAVDYPELAELWTRFASLPIRNAGTLGGNVANGSPIGDSMPALLA
LGAEVVLRHGPKTRSLPLDAFCLGYQKTALAAGEFVAALRVPRPAGNLRFRTYKVSKRYD
QDISAVCAAFALHLGEHGVIEQARIAFGGMAATPKRAAQTEAVLNGASWDESTARQAMNA
LVADYQPLTDMRASSAYRLKVARNLLWRFHLETRDDAPLALFDVNAFAFEAGAPDAAVAK
EPS