Protein Info for BPHYT_RS14995 in Burkholderia phytofirmans PsJN

Annotation: formate dehydrogenase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 982 PF13510: Fer2_4" amino acids 49 to 123 (75 residues), 89.5 bits, see alignment 4.9e-29 PF10588: NADH-G_4Fe-4S_3" amino acids 130 to 169 (40 residues), 59.1 bits, see alignment (E = 9.5e-20) PF12838: Fer4_7" amino acids 195 to 252 (58 residues), 33.6 bits, see alignment 1.7e-11 PF00037: Fer4" amino acids 233 to 252 (20 residues), 24.5 bits, see alignment (E = 7e-09) PF04879: Molybdop_Fe4S4" amino acids 267 to 318 (52 residues), 59.6 bits, see alignment 9.7e-20 TIGR01591: formate dehydrogenase, alpha subunit" amino acids 271 to 946 (676 residues), 938.6 bits, see alignment E=8.7e-287 PF00384: Molybdopterin" amino acids 323 to 753 (431 residues), 247.9 bits, see alignment E=6.5e-77 PF01568: Molydop_binding" amino acids 839 to 943 (105 residues), 104.1 bits, see alignment E=1.8e-33

Best Hits

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 100% identity to bpy:Bphyt_3023)

Predicted SEED Role

"NAD-dependent formate dehydrogenase alpha subunit" in subsystem Formate hydrogenase

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T651 at UniProt or InterPro

Protein Sequence (982 amino acids)

>BPHYT_RS14995 formate dehydrogenase subunit alpha (Burkholderia phytofirmans PsJN)
MSDVLNSPTGGCGSGNCACKSQAQQQRAPRSFFDDTDFGTPERHADIDITLEIDGQSVTV
PAGTSVMRAAVEAGVNVPKLCATDSLEPFGSCRLCLVEIEGKRGYPASCTTPVEAGMKVR
TQTDRLQSLRRNVMELYISDHPLDCLTCPANGDCELQDMAGVTGLREVRYGFDGANHLKD
KKDESNPYFTYDASKCIVCNRCVRACEETQGTFALTIAGRGFESRVAASENVPFMESECV
SCGACVAACPTATLQEKTVIMLGQAEHSVVTTCAYCGVGCSFKAEMKGNEVVRMVPHKNG
QANEGHACVKGRFAWGYATHKDRITKPMIRAKITDPWREVSWEEALTYAASEFRRIQAKH
GRDSIGGITSSRCTNEETYLVQKLVRAAFGNNNVDTCARVCHSPTGYGLKTTLGESAGTQ
TFASVDKADVIMVIGANPTDGHPVFGSRLKRRVREGAKLIVVDPRRIDIVDTPHVKASHH
LQLRPGTNVAVVNALAHVIVTEGLVNEAFVAERCETRAFEQWRDFVALPENAPEATEAVT
GVPAQFVREAARIYATGGNAAIYYGLGVTEHAQGSTMVMGIANLAMATGNIGREGVGVNP
LRGQNNVQGSCDMGSFPHELPGYRHISDTVTRTLFEDAWNVTLQPEPGLRIPNMFDAAVH
GTFKGLYCQGEDIVQSDPNTHHVSAALSSMECIVVQDIFLNETAKYAHVLLPGSSFLEKD
GTFTNAERRISRVRKVMPPVPGYADWEVTVMLSRALGYEMDYTHPSQIMDEIARLTPTFH
GVSYKKLDEMGSIQWPCNEDAPDGTPTMHIDSFVRGKGKFVITKFIATPEKVTRKFPMLL
TTGRILSQYNVGAQTRRTENSRWHDEDRLEIHPHDAEERGIKTDDWVGIESRAGQTVLRA
KVTERMQPGVVYTTFHFPESGANVITTDSSDWATNCPEYKVTAVQVMPVEQPSQWQKEYS
RFNTEQLDLLKQRELANATSGK