Protein Info for BPHYT_RS14535 in Burkholderia phytofirmans PsJN

Annotation: nicotinate phosphoribosyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01514: nicotinate phosphoribosyltransferase
  • amino acids 2 to 388 (387 residues), 470.9 bits, E=1.8e-145
• PF17767: NAPRTase_N
  • amino acids 7 to 128 (122 residues), 131.5 bits, E=2.2e-42
• PF04095: NAPRTase
  • amino acids 164 to 389 (226 residues), 242.9 bits, E=4.1e-76

Best Hits

Swiss-Prot: 89% identical to PNCB_BURCC: Nicotinate phosphoribosyltransferase (pncB) from Burkholderia cenocepacia (strain MC0-3)

KEGG orthology group: K00763, nicotinate phosphoribosyltransferase [EC: 2.4.2.11] (inferred from 100% identity to bpy:Bphyt_2932)

Predicted SEED Role

"Nicotinate phosphoribosyltransferase (EC 2.4.2.11)" in subsystem NAD and NADP cofactor biosynthesis global or NAD regulation or Redox-dependent regulation of nucleus processes (EC 2.4.2.11)

MetaCyc Pathways

• NAD salvage pathway I (PNC VI cycle) (5/7 steps found)
• NAD salvage pathway V (PNC V cycle) (3/5 steps found)
• superpathway of NAD biosynthesis in eukaryotes (9/14 steps found)
• NAD salvage (plants) (5/11 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Nicotinate and nicotinamide metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.11

Use Curated BLAST to search for 2.4.2.11

Sequence Analysis Tools

See B2T5W2 at UniProt or InterPro

Protein Sequence (398 amino acids)

>BPHYT_RS14535 nicotinate phosphoribosyltransferase (Burkholderia phytofirmans PsJN)
MIITSLLDTDLYKFTMMQVVLHHFPAANVEYRFRCRTPNVDLVPYIGEIRDEVRKLCQLR
FTDDELDYLRRMRFIKGDFIEFLALFHLNEKYISIEPSPKGNGEIDIEIKGPWLHTILFE
IPMLAIVNEVYFRNTQQKPDYSEGRGRLVDKIQLLGARPEFADCKIADYGTRRRFSKQWH
EEVILKLKEGLGEQFAGTSNVFYAMKHSLTPLGTMAHEYLQACQALGPRLRDSQIYGFEM
WAKEYRGDLGIALSDVYGMEAFLRDFDMYFCKLFDGARHDSGDPFDWGERLLKHYEANRC
DPRTKILVFSDALDIPKVLQLYERFRGRCKLAFGVGTNLTNDLGYNPLQIVIKMVRCNGQ
PVAKLSDSPGKNMCEDKAYLAYLRQVFGIAQPEEEAAK