Protein Info for BPHYT_RS14395 in Burkholderia phytofirmans PsJN

Annotation: sugar dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 347 signal peptide" amino acids 1 to 35 (35 residues), see Phobius details PF03888: MucB_RseB" amino acids 46 to 217 (172 residues), 211.1 bits, see alignment E=1.4e-66 PF17188: MucB_RseB_C" amino acids 242 to 342 (101 residues), 96.2 bits, see alignment E=1.7e-31

Best Hits

KEGG orthology group: K03598, sigma-E factor negative regulatory protein RseB (inferred from 100% identity to bpy:Bphyt_2904)

Predicted SEED Role

"Sigma factor RpoE negative regulatory protein RseB precursor" in subsystem Transcription initiation, bacterial sigma factors

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SZW4 at UniProt or InterPro

Protein Sequence (347 amino acids)

>BPHYT_RS14395 sugar dehydratase (Burkholderia phytofirmans PsJN)
MQTPRLNKTTIWGRLPAFLFCAAVLLSATPRVFAQTDDPIVARRTAADLLDRIHQAAQQQ
NYEGAFVYQRGNFVQTSRIAHYATRSDGEFEQLESLDGKPRKMLRHNDELYTFVPERHLC
VVEKRQNKDSFPALLAVSGEQVLSVYEPKLLGDDRVAGVDSQVIELDPKDAYRFAYKLWA
DKKTGLLLRAQTLDTSGQVLEQLSFSQIRIGVPVDKTGIVNGIRNTAGWTVVRPPVEPVD
MAAQGWQITPTVPGFRKIRELRRPMAARDPGQPTIPVDQAVFSDGLAAISVFVEPVENNT
RKEGAGSSGATHVLVKRRGDFWITLLGEVPQTTLQQFASAIEYKAPK