Protein Info for BPHYT_RS13980 in Burkholderia phytofirmans PsJN
Annotation: alanine--tRNA ligase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 91% identical to SYA_BURTA: Alanine--tRNA ligase (alaS) from Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CIP 106301 / E264)
KEGG orthology group: K01872, alanyl-tRNA synthetase [EC: 6.1.1.7] (inferred from 91% identity to bmj:BMULJ_01335)Predicted SEED Role
"Alanyl-tRNA synthetase (EC 6.1.1.7)" (EC 6.1.1.7)
MetaCyc Pathways
- tRNA charging (20/21 steps found)
- superpathway of chorismate metabolism (42/59 steps found)
- shinorine biosynthesis (3/8 steps found)
- vanchrobactin biosynthesis (2/8 steps found)
- enterobactin biosynthesis (4/11 steps found)
- ergotamine biosynthesis (4/12 steps found)
- (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
- bacillibactin biosynthesis (3/12 steps found)
- superpathway of ergotamine biosynthesis (5/18 steps found)
- sulfazecin biosynthesis (1/16 steps found)
- phosalacine biosynthesis (6/25 steps found)
- phosphinothricin tripeptide biosynthesis (6/25 steps found)
- corallopyronin A biosynthesis (2/30 steps found)
- colibactin biosynthesis (6/38 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.1.1.7
Use Curated BLAST to search for 6.1.1.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See B2SZN0 at UniProt or InterPro
Protein Sequence (874 amino acids)
>BPHYT_RS13980 alanine--tRNA ligase (Burkholderia phytofirmans PsJN) MKAAEIREKFLKFFESKGHTIVRSSSLVPGNDPTLLFTNSGMVQFKDVFLGAESRPYSRA TTAQRSVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKRDAIHYAWELLTGVYQLP KDKLWVTVYHEDDEAHDIWAKEVGVPVERIIRIGDNKGARYASDNFWQMADVGPCGPCSE IFYDHGPDVWGGPPGSPEEDGDRYIEIWNLVFMQFSRDAQGNMTPLPKQCVDTGMGLERI AAVLQHVHSNYEIDLFQALIKAAGRETGVSDLTNNSLKVIADHIRACSFLIVDGVIPGNE GRGYVLRRIVRRAIRHGYKLGKKGSFFHRMVADLVAQMGDAYPELKEAEQRVTDVLRQEE ERFFETIEHGMSILESALADLEAKGGKTLDGELAFKLHDTYGFPLDLTADVCREREVTVD EAAFDEAMARQREQARAAGKFKMAQGLEYSGAKTTFHGYEEIVFDDAKVIALYVDGASVK EVRDGRQAVVVLDHTPFYAESGGQVGDQGVLANASVRFAVSDTLKVQADVVGHHGTLEQG TLKVGDVVKAEIDAVRRARTARNHSATHLMHKALREVLGSHVQQKGSLVDADKTRFDFAH NAPMTDEQIRRVEEIVNAEVLANAPGIVRVMPFDEAVKGGAMALFGEKYGDEVRVLDLGF SRELCGGTHVHRTGDIGLFKIVMEGGVAAGIRRVEAITGDNAVRFVQDLDARINAAAAVL KAQPSELTQRITQVQDQVKSLEKELSALKSKMASSQGDELAGQAIEVGGVHVLAATLEGA DVKTLRETVDKLKDKLKSAAIVLASVEGGKVSLIAGVTADASKKVKAGELVNFVAQQVGG KGGGRPDMAQAGGTEPANLPAALAGVKGWVEGQL