Protein Info for BPHYT_RS13835 in Burkholderia phytofirmans PsJN

Annotation: S-adenosylhomocysteine nucleosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01048: PNP_UDP_1
  • amino acids 67 to 310 (244 residues), 168.3 bits, E=9.6e-54
• TIGR01704: MTA/SAH nucleosidase
  • amino acids 69 to 301 (233 residues), 114.2 bits, E=3.3e-37

Best Hits

KEGG orthology group: K01243, S-adenosylhomocysteine/5'-methylthioadenosine nucleosidase [EC: 3.2.2.9] (inferred from 100% identity to bpy:Bphyt_2796)

Predicted SEED Role

"5'-methylthioadenosine nucleosidase (EC 3.2.2.16) @ S-adenosylhomocysteine nucleosidase (EC 3.2.2.9)" (EC 3.2.2.16, EC 3.2.2.9)

MetaCyc Pathways

• S-methyl-5'-thioadenosine degradation IV (1/1 steps found)
• S-adenosyl-L-methionine salvage I (3/4 steps found)
• autoinducer AI-2 biosynthesis II (Vibrio) (4/6 steps found)
• S-methyl-5'-thioadenosine degradation I (1/2 steps found)
• autoinducer AI-2 biosynthesis I (3/5 steps found)
• 5'-deoxyadenosine degradation II (1/4 steps found)
• L-cysteine biosynthesis VI (reverse transsulfuration) (3/7 steps found)
• L-methionine salvage cycle I (bacteria and plants) (3/12 steps found)
• L-methionine salvage cycle II (plants) (2/11 steps found)

KEGG Metabolic Maps

• Methionine metabolism
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.2.16 or 3.2.2.9

Sequence Analysis Tools

See B2SZK2 at UniProt or InterPro

Protein Sequence (316 amino acids)

>BPHYT_RS13835 S-adenosylhomocysteine nucleosidase (Burkholderia phytofirmans PsJN)
MSGTGAGAGMSGVDAARQVAHGVETVGVEAQGVEAAGTPAVVGGSSAASLSAIDPDRSGK
PLSRRPLGILAALPQELGDLIEAMRAESGVRTITHGQRDYHVGTVHGAPCVVTLARVGKV
AAAATVSALIHAFDVEAVVFTGVAGGVGSEVRVGDIVVADALLQHDLDASPLFPRFEVPL
LGVSRFDADVALADQLAAACGRFVAEEGLALAARFGTREPRVHRGLIISGDQFVASAAGV
QALREALPDALAVEMEGAAIAQVCYEYGVPCAVVRTVSDTADDHAPASFVSFLTEIAGTY
SNAILTRFLEARSSTV