Protein Info for BPHYT_RS13710 in Burkholderia phytofirmans PsJN

Annotation: glycerol acyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF07690: MFS_1
  • amino acids 39 to 336 (298 residues), 49.1 bits, E=6.1e-17
• PF05977: MFS_3
  • amino acids 73 to 333 (261 residues), 32.6 bits, E=4.9e-12
• PF01553: Acyltransferase
  • amino acids 460 to 587 (128 residues), 99.2 bits, E=2.5e-32

Best Hits

KEGG orthology group: K00655, 1-acyl-sn-glycerol-3-phosphate acyltransferase [EC: 2.3.1.51] (inferred from 100% identity to bpy:Bphyt_2768)

Predicted SEED Role

"Lysophospholipid transporter LplT / 2-acylglycerophosphoethanolamine acyltransferase (EC 2.3.1.40) / Acyl-[acyl-carrier-protein] synthetase (EC 6.2.1.20)" (EC 2.3.1.40, EC 6.2.1.20)

MetaCyc Pathways

• phosphatidylglycerol biosynthesis I (6/6 steps found)
• phosphatidylglycerol biosynthesis II (6/6 steps found)
• superpathway of phospholipid biosynthesis III (E. coli) (10/12 steps found)
• CDP-diacylglycerol biosynthesis I (4/4 steps found)
• CDP-diacylglycerol biosynthesis II (4/4 steps found)
• oleate biosynthesis III (cyanobacteria) (2/3 steps found)
• superpathway of cardiolipin biosynthesis (bacteria) (9/13 steps found)
• palmitoleate biosynthesis III (cyanobacteria) (1/2 steps found)
• phospholipid remodeling (phosphatidate, yeast) (1/2 steps found)
• CDP-diacylglycerol biosynthesis III (3/5 steps found)
• phosphatidate biosynthesis (yeast) (3/5 steps found)
• alkane biosynthesis I (1/3 steps found)
• heptadecane biosynthesis (1/3 steps found)
• diacylglycerol and triacylglycerol biosynthesis (3/7 steps found)
• palmitoyl ethanolamide biosynthesis (2/6 steps found)
• superpathway of stearidonate biosynthesis (cyanobacteria) (2/6 steps found)
• stigma estolide biosynthesis (2/7 steps found)
• anandamide biosynthesis II (2/8 steps found)
• mycobactin biosynthesis (3/11 steps found)
• anandamide biosynthesis I (3/12 steps found)
• plasmalogen biosynthesis I (aerobic) (4/16 steps found)
• superpathway of phospholipid biosynthesis II (plants) (11/28 steps found)

KEGG Metabolic Maps

• Fatty acid metabolism
• Glycerolipid metabolism
• Glycerophospholipid metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.51

Use Curated BLAST to search for 2.3.1.40 or 2.3.1.51 or 6.2.1.20

Sequence Analysis Tools

See B2SZH4 at UniProt or InterPro

Protein Sequence (645 amino acids)

>BPHYT_RS13710 glycerol acyltransferase (Burkholderia phytofirmans PsJN)
MSDRPLPAARRAARQHEAHESQFRLLNQRRFAPFFWTQFLGAMNDNVFKIGFTSLVTYQA
ARFSGVDPKTAAFLISAIFILPFVLLSATSGQIADKYDKAMLTRFVKSFEIVVMLIGGAG
FWLHSAPLLYLCTFLMGVHSTVFGPVKYSYLPQHLSKAELVGGNGMVEMGTFVAILIGTI
VGGVGAGFAEHGAVVLACACVAIALVGRVVSSFVPVTPAPQADLRINWNPVSETWRNLKL
ARENRTVFLSLLGISWLWFVGAIFLSSFFSFAKNVLSADPDVVTVLLGTFSIGIGLGSLM
CEKLSKRRIEIGLVPLGSIGMSVFAIDLFFASHALPLPGHLLSVGEFIVRPAHWRVLADL
FLLAMFGGFYSVPLYALIQSRSQPSHRARIIAANNILNSLFMIVSSLMAVALTSAGFSIP
AIFLVTALLNIVVATYIYSLVPEFLLRFIAWVLVHTFYRIRLVHAERIPEEGAAVLVCNH
VSFVDAIVIMAESPRPIRFVMDHQIFKSPFAGWVFRHAKAIPIAPAHQDPQVLTRAYERC
AQALAEGDLVCIFPEGKLTRTGDMNPFRHGVTEIIRRTPAPVIPMALRGLWGSVFSRAGD
ARWPRPIQKGVMSRLTLAVGEPIEPELATPETLQQIVTELRGARK