Protein Info for BPHYT_RS13655 in Burkholderia phytofirmans PsJN

Annotation: GTP pyrophosphokinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 747 PF13328: HD_4" amino acids 19 to 186 (168 residues), 165.5 bits, see alignment E=2e-52 TIGR00691: RelA/SpoT family protein" amino acids 25 to 741 (717 residues), 565.8 bits, see alignment E=6.6e-174 PF04607: RelA_SpoT" amino acids 245 to 355 (111 residues), 150.7 bits, see alignment E=4.5e-48 PF02824: TGS" amino acids 413 to 471 (59 residues), 75.3 bits, see alignment 7.8e-25 PF13291: ACT_4" amino acids 669 to 745 (77 residues), 58.3 bits, see alignment E=2.5e-19

Best Hits

KEGG orthology group: K00951, GTP pyrophosphokinase [EC: 2.7.6.5] (inferred from 100% identity to bpy:Bphyt_2757)

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.5

Use Curated BLAST to search for 2.7.6.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SZG3 at UniProt or InterPro

Protein Sequence (747 amino acids)

>BPHYT_RS13655 GTP pyrophosphokinase (Burkholderia phytofirmans PsJN)
MNTETVTNAPNPLPSFDEAMAFVREHAGEVRLSSGEMLADHAAGTASIMRKLNVDPPAVL
AAALFALTPHLQDPERVIADNFGEEVAQLVGDVRKLLRLGTVSLRAAQNAMPEAGRDAQA
ARRAQVEALRKMLLAFAQDIRVVLIRLASRLQSLRYYAAAKITPSPDVARETLDIYAPLA
NRLGIWQLKWELEDLALRFEEPVTYKRIAKLLDEKRVERESYVAQAIERLQKELAAANIR
AEVSGRPKHIYSIWRKMRGKELDFAELYDVRAFRVIVPDIKDCYTVLGIVHNLWQPVPKE
FDDYISRPKPNGYKSLHTVVIGDDGRAFEVQIRTQEMHQFAEYGVAAHWRYKEAGTRGYG
GQFAANEKYDEKIAWLRQLLAWKDEVSEGEHGEKRASQPWEQLRQATLDDDHIYVLTPQA
RVIPLPHGATPVDFAYHLHSELGHRCRGARVDGAMVPLNTPLQNGQTVEIVAVKEGGPSR
DWLNPQLGYLQSPRARQKVRAWFNAVELQEHIASGRAMVEKTLQREGKTSVNLDQLAAKL
GFKTTDDLFSVVGKEEFSLRLVEQALHDAPPPEPVVEAPEQFEKRSSGASVARGASTGVL
VVGVDALLTQLARCCRPAPPDDICGFVTRGKGMSIHRSDCPTFLRMADRAPERVLQTAWS
AEVMSGRGQSVYPVDLSIDATDRQGLLRDISEVFAREKMNVIGVKTQTRRNAAFMQFTVE
VSSAAQIQRACTLLGEVTGVVRASRKS