Protein Info for BPHYT_RS13645 in Burkholderia phytofirmans PsJN

Annotation: threonine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 635 PF02824: TGS" amino acids 4 to 61 (58 residues), 34.6 bits, see alignment 3.2e-12 TIGR00418: threonine--tRNA ligase" amino acids 72 to 630 (559 residues), 743.5 bits, see alignment E=1e-227 PF07973: tRNA_SAD" amino acids 169 to 218 (50 residues), 61.1 bits, see alignment 1.7e-20 PF00587: tRNA-synt_2b" amino acids 317 to 528 (212 residues), 168.5 bits, see alignment E=3.2e-53 PF03129: HGTP_anticodon" amino acids 540 to 629 (90 residues), 75.5 bits, see alignment E=6e-25

Best Hits

Swiss-Prot: 100% identical to SYT_PARPJ: Threonine--tRNA ligase (thrS) from Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)

KEGG orthology group: K01868, threonyl-tRNA synthetase [EC: 6.1.1.3] (inferred from 100% identity to bpy:Bphyt_2756)

Predicted SEED Role

"Threonyl-tRNA synthetase (EC 6.1.1.3)" (EC 6.1.1.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.3

Use Curated BLAST to search for 6.1.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SZG2 at UniProt or InterPro

Protein Sequence (635 amino acids)

>BPHYT_RS13645 threonine--tRNA ligase (Burkholderia phytofirmans PsJN)
MVSIRLPDGSVRQYEHPVTVAEVAASIGPGLAKAALGGKIDGELVDTSALIDHDVALAIV
TEKDADGLDIIRHSTAHLLAYAVKDLYPEAQVTIGPVIDNGFYYDFAYNRPFTPEDLEKI
EKRMQELAKKDEPVSRRVVSRDEAVDYFKSIGEKYKAEIIESIPATDDIKLYSHGGFTDL
CRGPHVPSTGKLKVFKLMKVAGAYWRGDSKNEQLQRIYGTAWTKKEDQEAYLHMLEEAEK
RDHRKLGKQLDLFHMQDESPGMVFWHPRGWTLWQQVEQYMRRRVNDAGYLEIKTPMIMDR
SLWEASGHWQNYRENMFTTESEKRDYAIKPMNCPGHVQVFNHGLRSYRDLPLRYAEFGSC
HRNESSGALHGLMRVRGFVQDDAHIFCTEDQFISESIAFNTLAMSVYKDFGFDNVEIKLS
LRPDARAGTDETWDRAEQGLREALTACGVTWEELPGEGAFYGPKVEYHIKDALGRSWQCG
TLQLDMVLPERLGAEYVAEDNSRRRPIMLHRAIVGSMERFLGILIEHHAGAMPAWLAPMQ
VVVMNIAESQAEYAQSLAQSLQKQGVRVEADLRNEKISYKIREHTLEKVPYLLVVGDKER
EAQTVAVRARGGVDLGVMPPDTFIERLRQDVQSFN