Protein Info for BPHYT_RS13405 in Burkholderia phytofirmans PsJN

Annotation: phospholipase C

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 703 signal peptide" amino acids 1 to 36 (36 residues), see Phobius details TIGR03396: phospholipase C, phosphocholine-specific" amino acids 5 to 702 (698 residues), 907.3 bits, see alignment E=3.2e-277 PF04185: Phosphoesterase" amino acids 48 to 433 (386 residues), 410 bits, see alignment E=9.6e-127 PF05506: PLipase_C_C" amino acids 506 to 592 (87 residues), 99.1 bits, see alignment E=2.3e-32 amino acids 607 to 688 (82 residues), 61.2 bits, see alignment E=1.6e-20

Best Hits

Swiss-Prot: 69% identical to PHLN_BURPS: Non-hemolytic phospholipase C (plcN) from Burkholderia pseudomallei (strain K96243)

KEGG orthology group: K01114, phospholipase C [EC: 3.1.4.3] (inferred from 100% identity to bpy:Bphyt_2708)

Predicted SEED Role

"Phospholipase C 4 precursor (EC 3.1.4.3)" (EC 3.1.4.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.4.3

Use Curated BLAST to search for 3.1.4.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SY24 at UniProt or InterPro

Protein Sequence (703 amino acids)

>BPHYT_RS13405 phospholipase C (Burkholderia phytofirmans PsJN)
MTSKNRRDFLRSAAHAAGSATALSMLPLGIRNALAIPANNKTGTIRDVEHIVVLMQENRS
FDHYFGTLKGVRGFGDTRAINLPNGKPVWYQPLAADAGYVLPFRPSAPNLGLQFLQDLAH
DWTSTHAAWNGGRYDQWVPAKSATTMAYFTREDIPFHYQLADAFTICDAYHCSLMGPTDP
NRYYMWSGWVGNDGSGGGPVIDNSELGYGWSTYPEVLQNAGISWKIYQDIGTGLDANGSW
GWTQNPYIGNYGDNSLLYFNQYRNAQPGNPLYDNARTGTNAAKGDGYFDILKRDVQNNAL
PQVSWIVAPEAYSEHPNWPTNYGAWYIDQVLQILTSNPEVWSKTVLLINYDENDGFFDHM
VPPFAPSSSANGLSTVDTSNEIYPGDAKIPAAPYGLGPRVPMLVVSPWSKGGYVCSELFD
HTSVIRFIEKRFGHEHNLGESNITPWRRAVCGDLMSAFNFRNPNDAFPTLPGTSGYVPPD
QNRHPDYVPLPPALQAVPKQEPGVRPARALPYELFVRVHGEGSANQLTMRFVNTGRTGAV
FLVYAAHSLDAPRTYTVEAGKRLQDALPLNADGSYDFTVYGPNGFLRRFAGKPVARNWWN
GSDIARPEVEEGYDVANGNLQLRLENVGSAPSQFKIVNAYDPNNVIRHTVRGGDTDQIYL
DLRNAHGWYDLAITVNTDPLFARRFAGHVETGKSSMSDPALGS