Protein Info for BPHYT_RS13345 in Burkholderia phytofirmans PsJN

Annotation: crotonase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03222: benzoyl-CoA-dihydrodiol lyase
  • amino acids 11 to 556 (546 residues), 1017.4 bits, E=0
• PF00378: ECH_1
  • amino acids 52 to 241 (190 residues), 80.1 bits, E=8.8e-27

Best Hits

Swiss-Prot: 69% identical to BOXC_AZOEV: Benzoyl-CoA-dihydrodiol lyase (boxC) from Azoarcus evansii

KEGG orthology group: K01692, enoyl-CoA hydratase [EC: 4.2.1.17] (inferred from 100% identity to bpy:Bphyt_2696)

MetaCyc: 69% identical to benzoyl-CoA-dihydrodiol lyase monomer (Aromatoleum evansii)
RXN-11054 [EC: 4.1.2.44]

Predicted SEED Role

"Benzoyl-CoA-dihydrodiol lyase (EC 4.1.2.44)" (EC 4.1.2.44)

MetaCyc Pathways

• oleate β-oxidation (29/35 steps found)
• superpathway of phenylethylamine degradation (11/11 steps found)
• phenylacetate degradation I (aerobic) (9/9 steps found)
• adipate degradation (5/5 steps found)
• benzoyl-CoA degradation I (aerobic) (6/7 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• benzoyl-CoA biosynthesis (3/3 steps found)
• adipate biosynthesis (4/5 steps found)
• fatty acid β-oxidation I (generic) (5/7 steps found)
• valproate β-oxidation (6/9 steps found)
• fatty acid β-oxidation II (plant peroxisome) (3/5 steps found)
• fatty acid β-oxidation IV (unsaturated, even number) (3/5 steps found)
• 2-methyl-branched fatty acid β-oxidation (9/14 steps found)
• methyl ketone biosynthesis (engineered) (3/6 steps found)
• benzoate biosynthesis I (CoA-dependent, β-oxidative) (5/9 steps found)
• (8E,10E)-dodeca-8,10-dienol biosynthesis (6/11 steps found)
• fatty acid β-oxidation VI (mammalian peroxisome) (3/7 steps found)
• 3-phenylpropanoate degradation (5/10 steps found)
• benzoate biosynthesis III (CoA-dependent, non-β-oxidative) (1/5 steps found)
• (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (2/13 steps found)
• docosahexaenoate biosynthesis III (6-desaturase, mammals) (2/14 steps found)
• Spodoptera littoralis pheromone biosynthesis (4/22 steps found)
• platensimycin biosynthesis (6/26 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Biosynthesis of plant hormones
• Biosynthesis of unsaturated fatty acids
• Butanoate metabolism
• Caprolactam degradation
• Fatty acid elongation in mitochondria
• Fatty acid metabolism
• Geraniol degradation
• Limonene and pinene degradation
• Lysine degradation
• Propanoate metabolism
• Tryptophan metabolism
• Valine, leucine and isoleucine degradation
• alpha-Linolenic acid metabolism
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.17

Use Curated BLAST to search for 4.1.2.44 or 4.2.1.17

Sequence Analysis Tools

See B2SY12 at UniProt or InterPro

Protein Sequence (556 amino acids)

>BPHYT_RS13345 crotonase (Burkholderia phytofirmans PsJN)
MSTAETAVAPVDYRTEPSQYKHWKLSFNGPVATLGIDIAEDGGIRDGYKLKLNSYDLGVD
IELHDAVQRIRFEHPEVKTVVLTSLKDRVFCSGANIFMLGLSTHAWKVNFCKFTNETRNG
LEDSSRHSGLKFLAAVNGACAGGGYELALACDEIYLVDDRSSSVSLPEVPLLGVLPGTGG
LTRVTDKRKVRHDRADIFCTVVEGVRGERAKAWRLVDEVVKPNQFDQAIQARALELAEQS
DRPADAQGVVLTRIERTDREEGLTYKTLDVTIDRAKRIATFTAKAPQSEPPTNIDEIVAA
GVNWWPLQFARELDDAILCMRTNELAVGTWVFKTEGDARNLLAADASLMQHKDHWFVRET
IGLLRRTLARIDVSSRSLFALIEPGSCFAGTFAELAFAADRTYMAALPSNEEEEPAITLS
EVNFGLYPMVTHQSRLGRRFYEEAEPLDAVRSLIGQPIKPVEAERLGLVTASPDDIDWAD
EIRIALEERAAMSPDALTGLEANLRFNGPETMETRIFGRLTAWQNWIFNRPNAVGEKGAL
KVYGKGSKAQFDVSRV