Protein Info for BPHYT_RS13300 in Burkholderia phytofirmans PsJN

Updated annotation (from data): Dihydropyrimidinase (EC 3.5.2.2)
Rationale: Specifically important for utilizing Uridine. Automated validation from mutant phenotype: the predicted function (3.5.2.2) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: phenylhydantoinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02033: dihydropyrimidinase
  • amino acids 4 to 459 (456 residues), 582.1 bits, E=4.3e-179
• PF01979: Amidohydro_1
  • amino acids 54 to 415 (362 residues), 90.1 bits, E=1.7e-29

Best Hits

Swiss-Prot: 67% identical to HYDA_PSEAE: D-hydantoinase/dihydropyrimidinase (dht) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01464, dihydropyrimidinase [EC: 3.5.2.2] (inferred from 100% identity to bpy:Bphyt_2687)

MetaCyc: 47% identical to dihydropyrimidinase (Arabidopsis thaliana col)
Dihydropyrimidinase. [EC: 3.5.2.2]

Predicted SEED Role

"Dihydropyrimidinase (EC 3.5.2.2)" in subsystem Hydantoin metabolism or Pyrimidine utilization (EC 3.5.2.2)

MetaCyc Pathways

• thymine degradation (3/3 steps found)
• uracil degradation I (reductive) (3/3 steps found)
• superpathway of pyrimidine ribonucleosides degradation (4/5 steps found)

KEGG Metabolic Maps

• Drug metabolism - other enzymes
• Pantothenate and CoA biosynthesis
• Pyrimidine metabolism
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.2.2

Use Curated BLAST to search for 3.5.2.2

Sequence Analysis Tools

See B2SY03 at UniProt or InterPro

Protein Sequence (483 amino acids)

>BPHYT_RS13300 Dihydropyrimidinase (EC 3.5.2.2) (Burkholderia phytofirmans PsJN)
MTTLIRGGTIIDAENTYRADVLCADPQDGGTILQIGADLEAPAGATIVDAGGQYVMPGGI
DPHTHMELPFMGTTASDDFYTGTAAGLSGGTTSIIDFVIPSPKQPLMDAFKEWRGWAEKA
SSDYGFHVAVTWWDDSVYRDMGTLVHEHGVSSFKHFMAYKNAIMADDEVLVNSFSRSLEL
GALPTVHAENGELVFQLQRQLLAKGFTGPEAHPLSRPPEVEGEAANRAIRIAQVLGVPVY
IVHVSSKDAVDAIARARSEGLRVFGEVLPGHLVIDEAVYRDPDWTRAAAHVMSPPFRSAE
HREALWRGLQAGQLHTTATDHCVFCASQKAMGRDDFTKIPNGCGGVEDRMAVLWHHGVNS
GRLTPNEFVRITSTNAAQIFNLYPRKGAVRVGADADLVVWDPNASKTISVKTHHQKVDFN
VFEGMTVQGVAMHTLTRGALAWTDGELRAVRGAGRYLKRPPNGAYFDAIRVANKRKEPHP
VER