Protein Info for BPHYT_RS13220 in Burkholderia phytofirmans PsJN

Annotation: phosphomethylpyrimidine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 643 transmembrane" amino acids 489 to 504 (16 residues), see Phobius details PF13667: ThiC-associated" amino acids 21 to 98 (78 residues), 119.1 bits, see alignment E=5.8e-39 TIGR00190: phosphomethylpyrimidine synthase" amino acids 138 to 607 (470 residues), 713.9 bits, see alignment E=3e-219 PF01964: ThiC_Rad_SAM" amino acids 140 to 604 (465 residues), 616.8 bits, see alignment E=1.8e-189

Best Hits

Swiss-Prot: 100% identical to THIC_PARPJ: Phosphomethylpyrimidine synthase (thiC) from Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)

KEGG orthology group: K03147, thiamine biosynthesis protein ThiC (inferred from 100% identity to bpy:Bphyt_2670)

Predicted SEED Role

"Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17)" (EC 4.1.99.17)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.99.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SXY6 at UniProt or InterPro

Protein Sequence (643 amino acids)

>BPHYT_RS13220 phosphomethylpyrimidine synthase (Burkholderia phytofirmans PsJN)
MNANPKFLSADAHVDEAAVAPLPNSRKVYVTGSRADIRVPMREISQADTPDSFGGEKNPP
VYVYDTSGPYSDPEAKIDIRAGLPALRQAWIEERGDTEALTGLSSDFSRERAADTATADL
RFKGLHRTPRRAIAGKNVSQMHYARKGIITPEMEYIAIRENQRRAEYLESLKTSGPNGEK
LAAMMGRQHPGQAFGASAFGPNGLTAITPEFVREEVARGRAIIPNNINHPESEPMIIGRN
FLVKVNANIGNSAVTSSIGEEVDKMTWAIRWGGDTVMDLSTGKHIHETREWIIRNSPVPI
GTVPIYQALEKVNGKAEDLTWEIFRDTLIEQAEQGVDYFTIHAGVRLQYVPLTAKRMTGI
VSRGGSIMAKWCLAHHKESFLYEHFEDICEIMKAYDVAFSLGDGLRPGSIYDANDEAQLG
ELKTLGELTQIAWKHDVQTMIEGPGHVPMQLIKENMDLQLEWCDEAPFYTLGPLTTDIAP
GYDHITSGIGAAMIGWFGTAMLCYVTPKEHLGLPNKDDVKTGIITYKLAAHAADLAKGHP
GAQVRDNALSKARFEFRWEDQFNLGLDPDKAREFHDETLPKDSAKVAHFCSMCGPHFCSM
KITQDVREFAAQQGVTDDEALKKGMEVKSIEFMKKGAEIYQRQ