Protein Info for BPHYT_RS12980 in Burkholderia phytofirmans PsJN

Annotation: ATP-dependent helicase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1430 TIGR01967: RNA helicase HrpA" amino acids 117 to 1094 (978 residues), 1404 bits, see alignment E=0 PF00270: DEAD" amino acids 134 to 286 (153 residues), 25.8 bits, see alignment E=2.4e-09 PF00271: Helicase_C" amino acids 360 to 482 (123 residues), 45.4 bits, see alignment E=2.7e-15 PF04408: HA2_N" amino acids 546 to 572 (27 residues), 31.9 bits, see alignment (E = 3.1e-11) PF21010: HA2_C" amino acids 573 to 626 (54 residues), 35.2 bits, see alignment (E = 4.4e-12) PF07717: OB_NTP_bind" amino acids 697 to 773 (77 residues), 52.4 bits, see alignment 1.5e-17 PF11898: DUF3418" amino acids 788 to 1426 (639 residues), 713.9 bits, see alignment E=5.3e-218

Best Hits

Predicted SEED Role

"macromolecule metabolism; macromolecule synthesis, modification; dna - replication, repair, restr./modif."

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T5R5 at UniProt or InterPro

Protein Sequence (1430 amino acids)

>BPHYT_RS12980 ATP-dependent helicase (Burkholderia phytofirmans PsJN)
MSNVPKSPAAANENPAPAADQPKAGDAPRRDAQDAERNTPHTAREPRRAAENARARKNDS
NRGVRAQGNDATPKHAASDESRRASERGAGRREPRQPREPHAPRAPRAPRVVEPNPIPPI
TYPEALPVSGRREEIAKAIARNQVVIVCGETGSGKTTQLPKICLELGRGLGAGGSGLIGH
TQPRRIAASATGRRIAEELGTPFGEVVGYKVRFTDNLSPGASVKLMTDGILLAETQTDPL
LKAYDTLIIDEAHERSLNIDFLLGYLKEILVKRPDLKLIVTSATIDADRFARHFGSDEKP
APVIEVSGRLYPVEVRYRPVAEDSPAVKAAQGDAPSAPRGDRPKSQRETDRDLMDAIVDA
VDELCREGPGDVLVFLPGEREIRDAAEALRKHHPPHTEILPLFARLSAAEQERVFRTSNA
RRIVLATNVAETSLTVPGIRYVVDTGLARVKRYSYRNKVEQLQVESISQAAANQRAGRCG
RVADGICIRLYEESDYQSRARFTDPEILRSSLASVILRMKSLHLTAIETFPFIEPPPGRA
IADGYQLLNELGAVDDDNQLTPLGRELARLPLDPRVGRMILAARDQQALKEVLIIASALS
VQDPRDRPIEAQEQADQAHRRFADDRSEFLQWLKIWNWFEEAIAHKKSNKQLHEECRKNF
LSQLRLREWRDVHSQLLTVVREHGWRLNEAEATFEQIHLALLTGLLGNIGLKADDEPYYL
GARGIKFYLWPGSALVKKAGKWVMAAELVETSRLYARCIAKIEPEWIEKIGAHLLKKSLS
EPHWEKRAAQVSAFERAVLYGLPIYHRRRVSFGKQDPARARELFIRGALVEGEFDTKLAF
FAHNRKLLADIEQLEHKSRRQDVLVDDELIFAYYDQALPKGIYTGASFERWYRDEVKKSG
QPEDKLRLLYLSRDDLMRHEAAGVTTDLFPKRMTMAGVEMALTYHFEPGSPRDGVTLAVP
LYALNQVDARRCEWLVPGMLKEKTQLLLKSLPQKLRRHCVPLPEYAAGFVDRHSGPRFGA
GGLLESLIADVREQTQVAMKQSDFKLETLPPHLFMNFKVVDEHGRQLAMGRNMSQLRAEL
GGQAQQHFQKIASSAAGAALADAGGGGVATPAQTTGAAGAGALRGKGGVGAASGPQTAPQ
GGAQGTALYERLTTWNFGKLPELLEIRRGGQTLFGYPALVDRGTHCDVEVFDSPDEAARI
HRAGLRRLFALQLKEPIKYLEKNLPGLREMAMQFMPRGTQEELRDQLIDTALDRACLQDP
LPDDDVSFHTRRDEGRSRLTLLAQEIARLVGQILGEYASVAKKLVQAKSFTAAYADLQNQ
LDGLIGKRFVVDTPYAQLAHFPRYLKGIALRIDKLKADPARDARQFAEFQPLLQNYQRAV
AQRGGVLDPRLSEFRWLLEELRISLFAQELRTPMPISVKRLYKVWESMQR