Protein Info for BPHYT_RS12775 in Burkholderia phytofirmans PsJN

Annotation: FeS cluster assembly scaffold IscU

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01592: NifU_N
  • amino acids 2 to 126 (125 residues), 180 bits, E=1.1e-57
• TIGR01999: FeS cluster assembly scaffold IscU
  • amino acids 3 to 125 (123 residues), 226.8 bits, E=2.7e-72

Best Hits

Swiss-Prot: 82% identical to ISCU_ECOL6: Iron-sulfur cluster assembly scaffold protein IscU (iscU) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K04488, nitrogen fixation protein NifU and related proteins (inferred from 100% identity to bpy:Bphyt_2578)

MetaCyc: 82% identical to scaffold protein for iron-sulfur cluster assembly (Escherichia coli K-12 substr. MG1655)
RXN-14381

Predicted SEED Role

"Iron-sulfur cluster assembly scaffold protein IscU" in subsystem Wyeosine-MimG Biosynthesis

MetaCyc Pathways

• [2Fe-2S] iron-sulfur cluster biosynthesis (3/10 steps found)

Sequence Analysis Tools

See B2T5M1 at UniProt or InterPro

Protein Sequence (138 amino acids)

>BPHYT_RS12775 FeS cluster assembly scaffold IscU (Burkholderia phytofirmans PsJN)
MAYSDKVLDHYENPRNVGSFAKDDDAVGTGMVGAPACGDVMKLQIRVGADGIIEDAKFKT
YGCGSAIASSSLVTEWVKGKTLDQAMSIKNTQIAEELALPPVKIHCSILAEDAIKAAVAD
YKQRHGEAASVEGDKQHA