Protein Info for BPHYT_RS12550 in Burkholderia phytofirmans PsJN

Annotation: GTP-binding protein HflX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03156: GTP-binding protein HflX
  • amino acids 4 to 356 (353 residues), 490.8 bits, E=9.8e-152
• PF13167: GTP-bdg_N
  • amino acids 17 to 105 (89 residues), 108.7 bits, E=5.3e-35
• PF16360: GTP-bdg_M
  • amino acids 107 to 185 (79 residues), 106 bits, E=3.8e-34
• PF02421: FeoB_N
  • amino acids 192 to 354 (163 residues), 31 bits, E=5.1e-11
• PF01926: MMR_HSR1
  • amino acids 193 to 312 (120 residues), 76.4 bits, E=5.7e-25

Best Hits

Swiss-Prot: 56% identical to HFLX_ECOLI: GTPase HflX (hflX) from Escherichia coli (strain K12)

KEGG orthology group: K03665, GTP-binding protein HflX (inferred from 100% identity to bpy:Bphyt_2535)

MetaCyc: 56% identical to ribosome rescue factor HflX (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"GTP-binding protein HflX" in subsystem Hfl operon or Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Sequence Analysis Tools

See B2SXS4 at UniProt or InterPro

Protein Sequence (390 amino acids)

>BPHYT_RS12550 GTP-binding protein HflX (Burkholderia phytofirmans PsJN)
MINAALVGIDFGKIDFEASLEELSLLAQSAGANPLVTLTGRRSSPDAKMFVGSGKAEELR
LACEANDIELVIFNHALAPAQQRNLEQALNRRVIDRTSLILDIFAQRARSHEGKLQVELA
QLQYLSTRLIRAWTHLERQKGGIGLRGPGETQLETDRRLIGERIKALKTRLEKLRRQHGT
QRRARSRNQTMSVSLVGYTNAGKSTLFNALTKAQAYAADQLFATLDTTSRRVYLGDEAGQ
VVVSDTVGFIRELPHQLVAAFRATLEETIHADLLLHVVDASSAVRLDQIDQVNEVLHAIG
ADTIRQVLVFNKIDAVPELAARGDAVERDEYGNISRVFLSARTGQGLDTLRAAIAEIATA
EPLPETLVDLSEEDRSAAPRDDRKVPELGH