Protein Info for BPHYT_RS12545 in Burkholderia phytofirmans PsJN

Annotation: membrane protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 466 transmembrane" amino acids 88 to 109 (22 residues), see Phobius details PF12221: HflK_N" amino acids 18 to 71 (54 residues), 33 bits, see alignment 4.6e-12 TIGR01933: HflK protein" amino acids 106 to 379 (274 residues), 260.2 bits, see alignment E=9.9e-82 PF01145: Band_7" amino acids 108 to 293 (186 residues), 87.3 bits, see alignment E=1.3e-28

Best Hits

KEGG orthology group: K04088, membrane protease subunit HflK [EC: 3.4.-.-] (inferred from 100% identity to bpy:Bphyt_2534)

Predicted SEED Role

"HflK protein" in subsystem Hfl operon

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.-.-

Use Curated BLAST to search for 3.4.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SXS3 at UniProt or InterPro

Protein Sequence (466 amino acids)

>BPHYT_RS12545 membrane protein (Burkholderia phytofirmans PsJN)
MNDYNERSIWLRMRTMLSLNDPGWGRGDGNGDRQRPNEPKRPPTKDGEGPPDLDEMWRDF
NRRLSRVFGRKGGGAGGGRPDNGRSARIGVGIVIGVLLAIYLGSGVFVVQDGQAGVVMQF
GKYRYTAGQGVHWRLPYPFEAHELVNIGQIRQVEVGRNNVVRLANVKDASMLTHDADIVD
VRFAVQYQVKKPTDYLFRSVDPDQGVMQAAQAAVRSIVGARSTNDILYQDRETIRQQLMA
AIQQSLDEYQSGLAVTGVTIQGVQVPDRVQAAFDDAAKVRQENDRAKRDAEAYAADLLPR
AQADVARQIDEAKTYSDKTVAQAQGDAERFKQVYAQYSKAPAVVRERLYLDTMQQIYSNT
TKVYVDSKSGNNVLYLPLDKLVEQTRQRVADAAAASGTAAGATAAAAPQAASNAASPLSA
PSASATGAAASAPAASAAPASQAASGSDPLRSRDSFRSRMREDDVQ