Protein Info for BPHYT_RS12430 in Burkholderia phytofirmans PsJN

Annotation: cysteinyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 465 TIGR00435: cysteine--tRNA ligase" amino acids 4 to 464 (461 residues), 570.8 bits, see alignment E=1.3e-175 PF01406: tRNA-synt_1e" amino acids 17 to 318 (302 residues), 452.3 bits, see alignment E=1.8e-139 PF09334: tRNA-synt_1g" amino acids 37 to 111 (75 residues), 23.3 bits, see alignment E=5.1e-09 amino acids 250 to 303 (54 residues), 27.3 bits, see alignment 3.1e-10 PF09190: DALR_2" amino acids 347 to 402 (56 residues), 62.2 bits, see alignment 1.1e-20

Best Hits

Swiss-Prot: 96% identical to SYC_PARXL: Cysteine--tRNA ligase (cysS) from Paraburkholderia xenovorans (strain LB400)

KEGG orthology group: K01883, cysteinyl-tRNA synthetase [EC: 6.1.1.16] (inferred from 100% identity to bpy:Bphyt_2510)

MetaCyc: 56% identical to cysteine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Cysteine--tRNA ligase. [EC: 6.1.1.16]

Predicted SEED Role

"Cysteinyl-tRNA synthetase (EC 6.1.1.16)" in subsystem Conserved gene cluster possibly involved in RNA metabolism (EC 6.1.1.16)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.16

Use Curated BLAST to search for 6.1.1.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2SXP9 at UniProt or InterPro

Protein Sequence (465 amino acids)

>BPHYT_RS12430 cysteinyl-tRNA synthetase (Burkholderia phytofirmans PsJN)
MESLRIYNTLARDKQTFVPLQEGVVRMYVCGMTVYDYCHVGHARVMVVFDIVQRWLRTLG
YDVTYVRNITDIDDKIIRRAVENGETIKALTDRFIDALHEDADALGIQRPDLEPRATDFI
PQMLGMIEQLEANGYAYQASDGDVNYAVRKFANYGKLSGKSLEDLRAGERVAANDAKQDP
LDFVLWKQAKPDEPADTGWDSKYGRGRPGWHIECSAMGCTLLGEHFDIHGGGQDLQFPHH
ENEIAQSEAATGQTFVNFWMHNGYVQIDNEKMSKSLNNFFTIREVLAQYDAEVVRFFIAR
AHYRSPLNYSDVHIDDARNALARLYTALKDVTPDAAELDWNEAHAQRFQAAMNDDFNTPV
AVSVLFELATEVNRTRDPALARQLRSLGAVIGLLGREPRAYLQQAAGAAAEGALEPAAIE
AKIAARVAAKQAKDYAAADRIRAELLEAGVALEDKPGGSTEWRRV