Protein Info for BPHYT_RS12235 in Burkholderia phytofirmans PsJN

Annotation: DNA repair protein RadA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 458 TIGR00416: DNA repair protein RadA" amino acids 1 to 451 (451 residues), 641 bits, see alignment E=5.1e-197 PF18073: Rubredoxin_2" amino acids 8 to 35 (28 residues), 48.6 bits, see alignment (E = 1.7e-16) PF06745: ATPase" amino acids 70 to 254 (185 residues), 56.5 bits, see alignment E=9.7e-19 PF13481: AAA_25" amino acids 80 to 222 (143 residues), 55.1 bits, see alignment E=2.8e-18

Best Hits

Swiss-Prot: 63% identical to RADA_PSEAE: DNA repair protein RadA (radA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K04485, DNA repair protein RadA/Sms (inferred from 100% identity to bpy:Bphyt_2476)

Predicted SEED Role

"DNA repair protein RadA" in subsystem DNA repair, bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T5L5 at UniProt or InterPro

Protein Sequence (458 amino acids)

>BPHYT_RS12235 DNA repair protein RadA (Burkholderia phytofirmans PsJN)
MAKPKTLYICSECGGQSPKWAGQCPSCNAWNTLVESVAEAPSTHRFQALAKSAPVRRLAD
IEASDVPRFSTGVSEFDRVLGGGLVPGGVVLIGGDPGIGKSTLLLQSLAEIAADKRALYI
SGEESAAQIALRAQRLSLLEPGSKASELQLLAEIQLEKIQATISEQRPDVAVIDSIQTVY
SDALTSAPGSVAQVRECAAQLTRIAKQSGTTIIMVGHVTKEGALAGPRVLEHIVDTVLYF
EGDTHSSYRLVRAIKNRFGAVNELGVFAMTERGLRGVANPSALFLSQHEQSVPGSCVLVT
QEGTRPLLVEVQALVDSANAPNPRRLAVGLEQNRLAMLLAVLHRHAGIACFDQDVFLNAV
GGVKITEPAADLAVLLAIHSSMRNKPLPKGLVVFGEVGLAGEIRPSPRGQERLKEAAKLG
FSVAVIPKANAPKQPIEGLQVVAVERIEQAIDRVRTLE