Protein Info for BPHYT_RS12055 in Burkholderia phytofirmans PsJN

Annotation: phosphoenolpyruvate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01418: phosphoenolpyruvate synthase
  • amino acids 14 to 798 (785 residues), 1181.4 bits, E=0
• PF01326: PPDK_N
  • amino acids 26 to 354 (329 residues), 421.5 bits, E=3.9e-130
• PF00391: PEP-utilizers
  • amino acids 392 to 463 (72 residues), 97.1 bits, E=5.9e-32
• PF02896: PEP-utilizers_C
  • amino acids 489 to 798 (310 residues), 192.5 bits, E=1.4e-60

Best Hits

Swiss-Prot: 68% identical to PPSA_NEIMB: Phosphoenolpyruvate synthase (ppsA) from Neisseria meningitidis serogroup B (strain MC58)

KEGG orthology group: K01007, pyruvate, water dikinase [EC: 2.7.9.2] (inferred from 100% identity to bpy:Bphyt_2438)

MetaCyc: 65% identical to phosphoenolpyruvate synthetase (Escherichia coli K-12 substr. MG1655)
Pyruvate, water dikinase. [EC: 2.7.9.2]

Predicted SEED Role

"Phosphoenolpyruvate synthase (EC 2.7.9.2)" in subsystem Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes or Pyruvate metabolism I: anaplerotic reactions, PEP (EC 2.7.9.2)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (15/17 steps found)
• glycolysis II (from fructose 6-phosphate) (10/11 steps found)
• gluconeogenesis I (11/13 steps found)
• glycolysis I (from glucose 6-phosphate) (11/13 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (15/19 steps found)
• superpathway of anaerobic sucrose degradation (15/19 steps found)
• superpathway of N-acetylneuraminate degradation (17/22 steps found)
• glycolysis V (Pyrococcus) (7/10 steps found)
• superpathway of hexitol degradation (bacteria) (12/18 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (9/18 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (21/56 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 2.7.9.2

Use Curated BLAST to search for 2.7.9.2

Sequence Analysis Tools

See B2T5H7 at UniProt or InterPro

Protein Sequence (801 amino acids)

>BPHYT_RS12055 phosphoenolpyruvate synthase (Burkholderia phytofirmans PsJN)
MTNAVTVAKDKAYVVPFEQLRMTDVEIVGGKNASLGEMISQLAEAGVRVPTGFATTALAF
RDFLHHNNLTERIAKRLETLDVDDVKSLAEAGKEIRQWIVDAPFQPQLEADIRAGFDTLQ
NSSPEELSFAVRSSATAEDLPDASFAGQQESYLNVVGVEDVLDRMKHVFASLYNDRAISY
RVHKGFTHAEVALSAGVQRMVRSDVGAAGVMFTLDTESGFKDAVFITSSYGLGETVVQGA
VNPDEFYVYKTTLAQGKYPIIRRSIGSKLIKMEFTKAGEEGRVKTVDVAHEQRNRFSITD
EDVIELAKYAVIIEKHYERPMDIEWGKDGRDGKIFILQARPETVKSQAAGKAEQRFKLKG
QSNVLATGRAIGQKIGAGPVRVIHDPSEMDRVQPGDVLVADMTDPNWEPVMKRASAIVTN
RGGRTCHAAIIARELGVPAVVGCGDATDVLKEGALVTVSCAEGDEGRIYDGLLETEVTEV
QRGELPPIPVKIMMNVGNPQLAFDFSQLPNAGVGLARLEFIINNNIGVHPKAILEYPNID
QDLKKAVESVARGHASPRAFYVDKLTEGIATIAAAFYPKPVIVRLSDFKSNEYKKLIGGS
RYEPDEENPMLGFRGASRYIADDFAEAFQMECIALKKVREEMGLDNVEIMVPFVRTLKQA
ERVVGLLAKYGLKRGENGLRLIMMCEVPSNAILAEEFLQFFDGFSIGSNDLTQLTLGLDR
DSGMELLAVDFDERDPAIKFLLKRAIDTCLRLDKYVGICGQGPSDHPDFAQWLTDEGIKS
ISLNPDTIVETWQQLAKSAKQ