Protein Info for BPHYT_RS11755 in Burkholderia phytofirmans PsJN

Annotation: spermidine/putrescine ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 351 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details PF01547: SBP_bac_1" amino acids 42 to 287 (246 residues), 29 bits, see alignment E=1.1e-10 PF13416: SBP_bac_8" amino acids 47 to 313 (267 residues), 181.8 bits, see alignment E=2.5e-57

Best Hits

KEGG orthology group: K02055, putative spermidine/putrescine transport system substrate-binding protein (inferred from 100% identity to bpy:Bphyt_2372)

Predicted SEED Role

"ABC transporter, periplasmic spermidine putrescine-binding protein PotD (TC 3.A.1.11.1)" in subsystem Polyamine Metabolism (TC 3.A.1.11.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T5A9 at UniProt or InterPro

Protein Sequence (351 amino acids)

>BPHYT_RS11755 spermidine/putrescine ABC transporter substrate-binding protein (Burkholderia phytofirmans PsJN)
MRTLVKTQNVALAILAAAALTTVTTQAAETLSVVTFGGAYEAAAKKAYFEPFTQATGIGF
STESYDGGLAKLSAMEQAKNTTWDLIDLETNDAITACDEGLLQKFDKKTIGKTSDFIPGT
ISDCAVASMVWSTIYAYDASKLKSAPTTVNDFFDLQKFPGKRGLRKSPKVTMEWALIADG
VDPKDVYKVLGTPAGVDRAFKKLDTIKPNIVWWESGAQAPQLLADGAVVMTQAYNGRISG
AAKKDNKPFKTVWDAQVYDFDWWAVPTGAKHADAAAKFIASASQPKAYADLSKYIAYAPP
RKDAIALIDKQRLADLPTAPDNFKRALQINANFWADNADQINKRFQVWLTQ