Protein Info for BPHYT_RS11290 in Burkholderia phytofirmans PsJN

Updated annotation (from data): 2-ketogluconate 6-phosphate reductase (EC 1.1.1.43)
Rationale: Specifically important for: D-Gluconic Acid sodium salt. Gluconate is probably primarily catabolized via gluconate kinase (BPHYT_RS16720) and the Entner-Doudoroff pathway (starting with phosphogluconate dehydratase, BPHYT_RS16735). However it appears that there is a side pathway involving gluconate 2-dehydrogenase (BPHYT_RS14520, which has no phenotype because this side path is not beneficial), 2-ketogluconate kinase (BPHYT_RS11300, no fitness data), and 2-ketogluconate-6-P reductase (this gene). A similar side path is known in P. putida (PMCID:PMC4646247) and this gene is similar to the P. putida 2-ketoglutarate-6-phosphate reductase (PP3376). (SEED_correct)
Original annotation: bifunctional glyoxylate/hydroxypyruvate reductase B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00389: 2-Hacid_dh
  • amino acids 7 to 315 (309 residues), 97.8 bits, E=6.4e-32
• PF02826: 2-Hacid_dh_C
  • amino acids 104 to 283 (180 residues), 220.2 bits, E=2e-69
• PF03446: NAD_binding_2
  • amino acids 144 to 252 (109 residues), 23.3 bits, E=8.5e-09

Best Hits

Swiss-Prot: 59% identical to GHRB_YERE8: Glyoxylate/hydroxypyruvate reductase B (ghrB) from Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)

KEGG orthology group: K00090, gluconate 2-dehydrogenase [EC: 1.1.1.215] (inferred from 100% identity to bpy:Bphyt_2276)

MetaCyc: 55% identical to glyoxylate reductase (Escherichia coli K-12 substr. MG1655)
Glyoxylate reductase (NADP(+)). [EC: 1.1.1.79]; Gluconate 2-dehydrogenase. [EC: 1.1.1.79, 1.1.1.215]; 1.1.1.- [EC: 1.1.1.79, 1.1.1.215]; 1.1.1.- [EC: 1.1.1.79, 1.1.1.215]; 1.1.1.- [EC: 1.1.1.79, 1.1.1.215]

Predicted SEED Role

"2-ketogluconate 6-phosphate reductase (EC 1.1.1.43)" in subsystem 2-Ketogluconate Utilization (EC 1.1.1.43)

MetaCyc Pathways

• glucose degradation (oxidative) (5/5 steps found)
• ketogluconate metabolism (6/8 steps found)
• L-ascorbate degradation IV (2/5 steps found)

KEGG Metabolic Maps

• Glutathione metabolism
• Glyoxylate and dicarboxylate metabolism
• Pentose phosphate pathway
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.215, 1.1.1.79

Use Curated BLAST to search for 1.1.1.215 or 1.1.1.43 or 1.1.1.79

Sequence Analysis Tools

See B2T518 at UniProt or InterPro

Protein Sequence (321 amino acids)

>BPHYT_RS11290 2-ketogluconate 6-phosphate reductase (EC 1.1.1.43) (Burkholderia phytofirmans PsJN)
MKKIVAWKSLPEDVLAYLQQHAQVVQVDATQHDAFVAALKDADGGIGSSVKITPAMLEGA
TRLKALSTISVGFDQFDVADLTRRGIVLANTPDVLTESTADTVFSLILASARRVVELAEW
VKAGHWQHSIGPALFGVDVQGKTLGIVGLGRIGGAVARRAALGFNMKVLYTNRSANPQAE
EAYGARRVELAELLATADFVCLQVPLTPETKHLIGAAELKSMKKSAILINASRGATVDEK
ALIEALQNGTIHGAGLDVFETEPLPSDSPLLKLANVVALPHIGSATHETRHAMARNAAEN
LVAALDGTLTSNIVNREVLSK