Protein Info for BPHYT_RS10840 in Burkholderia phytofirmans PsJN

Annotation: 3-octaprenyl-4-hydroxybenzoate carboxy-lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00421: polyprenyl P-hydroxybenzoate and phenylacrylic acid decarboxylases
  • amino acids 10 to 192 (183 residues), 237 bits, E=5.9e-75
• PF02441: Flavoprotein
  • amino acids 10 to 180 (171 residues), 144.8 bits, E=9.4e-47

Best Hits

Swiss-Prot: 57% identical to UBIX_NEIMB: Flavin prenyltransferase UbiX (ubiX) from Neisseria meningitidis serogroup B (strain MC58)

KEGG orthology group: K03186, 3-octaprenyl-4-hydroxybenzoate carboxy-lyase UbiX [EC: 4.1.1.-] (inferred from 100% identity to bpy:Bphyt_2186)

MetaCyc: 78% identical to 2,5-furan-dicarboxylic acid decarboxylase 2 (Cupriavidus basilensis)
RXN-13093

Predicted SEED Role

"3-polyprenyl-4-hydroxybenzoate carboxy-lyase UbiX (EC 4.1.1.-)" in subsystem Ubiquinone Biosynthesis (EC 4.1.1.-)

MetaCyc Pathways

• superpathway of ubiquinol-8 biosynthesis (early decarboxylation) (10/12 steps found)
• ubiquinol-8 biosynthesis (early decarboxylation) (6/8 steps found)
• superpathway of chorismate metabolism (42/59 steps found)
• 5-hydroxymethylfurfural degradation (5/10 steps found)

KEGG Metabolic Maps

• 1,1,1-Trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) degradation
• 1- and 2-Methylnaphthalene degradation
• 3-Chloroacrylic acid degradation
• Ascorbate and aldarate metabolism
• Benzoate degradation via hydroxylation
• Biphenyl degradation
• Fluorene degradation
• Phenylalanine metabolism
• Phenylpropanoid biosynthesis
• Purine metabolism
• Pyruvate metabolism
• Tryptophan metabolism
• Tyrosine metabolism
• Ubiquinone and menaquinone biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 4.1.1.-

Use Curated BLAST to search for 4.1.1.-

Sequence Analysis Tools

See B2T4S7 at UniProt or InterPro

Protein Sequence (208 amino acids)

>BPHYT_RS10840 3-octaprenyl-4-hydroxybenzoate carboxy-lyase (Burkholderia phytofirmans PsJN)
MAEHAHKPQRIVVGISGASGAVIGVRLLAALRRLGTQETHLIVSASGAVTAAQELGMTRG
DLDSLADVVYNVRDIGAAVASGSFITAGMVIAPCSMKTLAGVANGFADNLLTRAADVMLK
ERRRLVLVARETPLNLAHLRNMTSVTEMGGIVMPPVPAFYAHPKTIEDVVDHTVGRILDL
FAIEHREIAQRWSGLADEFTERRSGVDE