Protein Info for BPHYT_RS10505 in Burkholderia phytofirmans PsJN
Annotation: 2-oxoglutarate dehydrogenase E1
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K00164, 2-oxoglutarate dehydrogenase E1 component [EC: 1.2.4.2] (inferred from 100% identity to bpy:Bphyt_2120)Predicted SEED Role
"2-oxoglutarate dehydrogenase E1 component (EC 1.2.4.2)" in subsystem TCA Cycle (EC 1.2.4.2)
MetaCyc Pathways
- 2-oxoglutarate decarboxylation to succinyl-CoA (3/3 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Citrate cycle (TCA cycle)
- Lysine degradation
- Tryptophan metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.2.4.2
Use Curated BLAST to search for 1.2.4.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See B2T4L0 at UniProt or InterPro
Protein Sequence (949 amino acids)
>BPHYT_RS10505 2-oxoglutarate dehydrogenase E1 (Burkholderia phytofirmans PsJN) MEDRFIMLMEKIQSSFLFGGNAPYVEEQYELYLADPASVADEWRAFFDALRETPAIDGSD RDDEPHAPVVSTFVDLARRSRATQITLDDGLAIARKQVAVQSLIAAYRMIGTRKAKLDPL LWTPPKASIELTPAFYGLSSADMSTRFSTADTFLFDEDATLQDLVAALEQTYTGTLGAEF MHLTDAGERRWWQMQLESTRAKPSFSVAEKHRFLERLTAAEGLEKYLHTRYVGQKRFSLE GGESLIVLLDELVRYGASKKVATVVMGMAHRGRLNVLVNIVGKPLRALFDEFEGKSGDSL PAGDVKYHKGYSSVTQTDDGPVDVVLAFNPSHLEIVNPVVQGMARAKGDASNERDGSDVL PVEIHGDAAMSGQGVVMETLSLSYTRGHGTGGTVHVVVNNQIGFTTSDPRDTRSSFYTTD IAKMIEAPVLHVNADDPEAVAMAVRLALDYRTTFRRSVVIDLVCFRRHGHQEQDTPAITQ PLMYRAIASHPGVRTVYAQQLIKEGVVTPDQVKDYLEAQRESFEHARESNAVEAADQTDE IPGWPKFLEDYAGPVQYGPPLSAQVRALAQQVSTVPDGYELHPLVGKMIAARREMAAGNK RLDWGMGEHMAFASLLSAGIDVRLSGQDSARGTFNHRHAVLHNQTRTSRSEGVYIPLEHI GEGQGRFTVTNSILSEAAVLAFEYGYSTVNRNALVVWEAQFGDFANGAQVVIDQFIAAGA AKWGQLSGLTLFLPHGQDGQGPEHASARLERYLQLSAQDNMRVVQPTTPAQLFHLLRMQA AVFDRRPLVVMTPKSLLRHPEAVSTLDDLATGAFREILVDTQAEPSPASSVERVIVSSGK VYFDLLEHRRTSGVADTPLIRVEQLYPFPSRQLAAEFARYPNLKTVVWCQEESRNQGAWS FVEPQLREILPSDAQLRYAGPAASASTAPGYHAVHAARQAALISSAFTD