Protein Info for BPHYT_RS10030 in Burkholderia phytofirmans PsJN

Annotation: hemolysin D

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 413 transmembrane" amino acids 28 to 49 (22 residues), see Phobius details PF16576: HlyD_D23" amino acids 67 to 287 (221 residues), 46.9 bits, see alignment E=5.2e-16 PF13533: Biotin_lipoyl_2" amino acids 68 to 114 (47 residues), 27 bits, see alignment 7.8e-10 PF00529: CusB_dom_1" amino acids 199 to 353 (155 residues), 35.2 bits, see alignment E=2.4e-12 PF13437: HlyD_3" amino acids 224 to 302 (79 residues), 52.8 bits, see alignment E=1.4e-17

Best Hits

Swiss-Prot: 48% identical to EMRA_ECOLI: Multidrug export protein EmrA (emrA) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_2021)

MetaCyc: 48% identical to multidrug efflux pump membrane fusion protein EmrA (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-363; TRANS-RXN-364; TRANS-RXN-365

Predicted SEED Role

"Membrane fusion component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T4B7 at UniProt or InterPro

Protein Sequence (413 amino acids)

>BPHYT_RS10030 hemolysin D (Burkholderia phytofirmans PsJN)
MSDTIKTSRTAAEADQPLQAQAPTKRKLLLSLLAATVVLAGAGYGAYYYTSGRYYQSTDD
AYVSGNLVQLTPQVSGTVVAVNADDTQIVKQGAPVVTLDNADAKVALSNAEATLGQTVRQ
VSGLYVNNDFYAATVAQRQSDLARANDDLKRRQAVAETGAVSGEDIAHARDAVSSAQAAL
DAARQQAEANHALTDRTSIEQHPNVQAAASKVRDAYLSYARNTLPAPVTGYVARRSVQVG
QRVAPGTPLMAIVPLDGVWVDANFKEGQLRHMRIGQPVTLTADVYGSSVKYHGRLEGFSA
GTGAAFASLPAQNATGNWIKVVQRLPARILLDQNELNAHPLRIGLSMDVEVDTHEETGPQ
LGAAINTSYHTDVFAQYGDEADAEIARIIEQNMPAKHELAASPTQKSTARKAG