Protein Info for BPHYT_RS09910 in Burkholderia phytofirmans PsJN

Annotation: tyramine oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02727: Cu_amine_oxidN2
  • amino acids 14 to 98 (85 residues), 34 bits, E=4.3e-12
• PF02728: Cu_amine_oxidN3
  • amino acids 109 to 213 (105 residues), 61.9 bits, E=9.3e-21
• PF01179: Cu_amine_oxid
  • amino acids 235 to 637 (403 residues), 533.2 bits, E=6.7e-164

Best Hits

Swiss-Prot: 47% identical to PAOX_ARTGO: Phenylethylamine oxidase from Arthrobacter globiformis

KEGG orthology group: K00276, primary-amine oxidase [EC: 1.4.3.21] (inferred from 100% identity to bpy:Bphyt_1997)

Predicted SEED Role

"Monoamine oxidase (1.4.3.4)" in subsystem Aromatic Amin Catabolism or Auxin biosynthesis or Glycine and Serine Utilization or Threonine degradation

MetaCyc Pathways

• superpathway of phenylethylamine degradation (11/11 steps found)
• superpathway of L-threonine metabolism (16/18 steps found)
• L-threonine degradation III (to methylglyoxal) (3/3 steps found)
• phenylethylamine degradation I (2/2 steps found)
• salidroside biosynthesis (3/4 steps found)
• L-phenylalanine degradation IV (mammalian, via side chain) (6/9 steps found)
• phenylethanol biosynthesis (3/5 steps found)
• aromatic biogenic amine degradation (bacteria) (4/8 steps found)

KEGG Metabolic Maps

• Alkaloid biosynthesis I
• Alkaloid biosynthesis II
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Glycine, serine and threonine metabolism
• Phenylalanine metabolism
• Tyrosine metabolism
• beta-Alanine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.3.21

Sequence Analysis Tools

See B2T496 at UniProt or InterPro

Protein Sequence (661 amino acids)

>BPHYT_RS09910 tyramine oxidase (Burkholderia phytofirmans PsJN)
MNTNATTAAHAPFHPLDPLSGAEMQLACDLVKAAEKLDSHARFPMVELREPPKAEVVAFK
TGEYFSRTAFVLAIDRTNGATIEFEVDLREKKIAARRVMPFGEAPYGQPPIMIDDFMNAE
QIVKSDEAWRVAVMKRGLSEKDLERVQVDPFSAGCFDRENENGRRLVRCVSYYRETLTDN
GYAHPIEGVMAVVDLLEKKVIELVDDGRIIPIPRAKHNYDTPSLGEPRSTLKPLSIDQPD
GPSFTIDGWHVNWQNWNFRVGFTPREGLVLHQLSWDDGKSTRPIIYRASVTEMCVPYSDP
TTNHYWKSAFDAGEYGLGKLANQLELGCDCLGTIRYFDIPSADDFGNPFVMKNAVCMHEE
DYGTLWKHYEFRTGVFEMRRSRRLVISFFATVGNYDYGFYWYLYQDGTIQLECKLTGIVQ
TSAVADGDTYPWGGMITENLGGPTHQHFFNARMHMMVDGERNTVTEHEFVPRPMGENNPY
GNVFDTTKRVLKTESEAARNANGSTGRYWKVSNPNVKNAVGANPGYKLVVNDSPLMLADE
RSKVRQRGGFATRHVWVTPFDPAERYASGDYPNQHSGGDGLPRYIEANRNIENEDVVLWH
SFGHTHVCKPEDFPVMPVEYAGFMLKPNNFFSANPTMDLPAERDLNSVEDGKSTDHGCCK
H