Protein Info for BPHYT_RS09875 in Burkholderia phytofirmans PsJN

Annotation: aldehyde dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 46% identical to ALDA_ECOLI: Lactaldehyde dehydrogenase (aldA) from Escherichia coli (strain K12)

KEGG orthology group: K07248, lactaldehyde dehydrogenase / glycolaldehyde dehydrogenase [EC: 1.2.1.21 1.2.1.22] (inferred from 100% identity to bpy:Bphyt_1990)

MetaCyc: 46% identical to aldehyde dehydrogenase A (Escherichia coli K-12 substr. MG1655)
Lactaldehyde dehydrogenase. [EC: 1.2.1.22]; Glycolaldehyde dehydrogenase. [EC: 1.2.1.22, 1.2.1.21]

Predicted SEED Role

"Aldehyde dehydrogenase A (EC 1.2.1.22)" in subsystem L-rhamnose utilization or Methylglyoxal Metabolism (EC 1.2.1.22)

MetaCyc Pathways

superpathway of glycol metabolism and degradation (5/7 steps found)
methylglyoxal degradation V (2/3 steps found)
L-lactaldehyde degradation (aerobic) (1/2 steps found)
ethylene glycol degradation (1/2 steps found)
L-rhamnose degradation II (5/8 steps found)
methylglyoxal degradation IV (1/3 steps found)
lactate biosynthesis (archaea) (2/5 steps found)
superpathway of methylglyoxal degradation (4/8 steps found)
D-arabinose degradation II (1/4 steps found)
superpathway of fucose and rhamnose degradation (4/12 steps found)
superpathway of pentose and pentitol degradation (22/42 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.22

Use Curated BLAST to search for 1.2.1.21 or 1.2.1.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T486 at UniProt or InterPro

Protein Sequence (481 amino acids)

>BPHYT_RS09875 aldehyde dehydrogenase (Burkholderia phytofirmans PsJN)
MRLDRNFANGRFIEPAADTASRELIAVCNPATEAVIAHVTGATQAEVIAAVDAAAVAQKG
WRKLPQAERAVYLHKLADALTECAPAIGAALALESGKSVADATNEAIYASQITRYHAEWA
RRIEGEVIPSDAPDENLVLHREPIGVVACLIPFNYPVYTFMRKVAPALIAGNTVVVRPSN
NTPTSAFEIAKAVEKAGLPAGVVNILAMNHATAEVLCTHPKVGMITLTGSVGAGRKVLDY
CKANIAKPSLELGGKTPAIIEADADLEKAARDLVASKTTHCGQLCTAIERVYVQESVHDR
FVALLKKHMSAVESGDRSEQPSLMGPLVNEASRQSIHAMVERAVAAGATLETGGKLPTPG
QGKGFFYPATLLSNCRQDMEIIQEETFGPVMPVVKYRTLDEALEMANDHQFGLSSVLYTE
NYRGAMKVANGIEAGELYVNRTPADPYQGFHAGWKRSGLGGDDGKHGMLEFTQTRLVVMK
Y