Protein Info for BPHYT_RS09850 in Burkholderia phytofirmans PsJN

Annotation: molybdopterin oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1403 PF04879: Molybdop_Fe4S4" amino acids 6 to 52 (47 residues), 71.9 bits, see alignment (E = 9.9e-24) PF00384: Molybdopterin" amino acids 81 to 487 (407 residues), 204.9 bits, see alignment E=5.7e-64 PF01568: Molydop_binding" amino acids 600 to 710 (111 residues), 119.5 bits, see alignment E=2.2e-38 PF00258: Flavodoxin_1" amino acids 870 to 1000 (131 residues), 97.7 bits, see alignment E=2.1e-31 PF00667: FAD_binding_1" amino acids 1035 to 1229 (195 residues), 106.1 bits, see alignment E=6.4e-34 PF00175: NAD_binding_1" amino acids 1261 to 1366 (106 residues), 62.8 bits, see alignment 1.3e-20

Best Hits

KEGG orthology group: None (inferred from 72% identity to bam:Bamb_3934)

Predicted SEED Role

"Assimilatory nitrate reductase large subunit (EC:1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.7.99.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T481 at UniProt or InterPro

Protein Sequence (1403 amino acids)

>BPHYT_RS09850 molybdopterin oxidoreductase (Burkholderia phytofirmans PsJN)
MSSTNVKTVCPYCGVGCGMVLHVEDGQVVKISGDKEHPANFGRLCTKGQSAHVALRKSGR
LEDAFVRHARGQDPAPLPMAQAISTTAARLRGLLDEHGPDALSFYVSGQMSIEAQYLVNK
LAKGFVGTSNIESNSRLCMASAGSGYKLSLGADGPPGSYEDIDHADLFFVIGANMADCHP
ILFLRMMDRVKAGAKLIVVDPRRNTTAEKADLFMQIKPGTDLALLNGLLHLLHENGHTDA
AFIADATEGWDAMPAFLADYTPENVAQITGLAVEDIRQAARMIGTAKEWISCWTMGLNQS
THGTWHTNAICNLHLATGKICRRGSGPFSLTGQPNAMGGREMGYMGPGLPGQRSVLVEED
RAFIEDLWGIPKDSLKPQVGNGTIDMFTRMAAGEIKACWIICTNPVASVANRQNVIDGLS
AAQLVIAQDAFLDTETNRYADVLLPGALWAEAEGVMINSERNLTLMQKGIEPPGAALPDW
QIIARVACEMGFADAFTYASAEEVFAEITRASNPKTGYDLRGASYRRLRETPLQWPLAPD
DSADRNPVRYLNDGVSQTLKERPDGSRPRLVFPTASGKAMFFARAYAAPAELPDREFPIV
LNTGRLQHQWHTMTKTGKVAMLNKLNPGPFVEIHPEDAATLGIKAKDPVEIRSRRGRAVL
PAVVTGRVNAGQCFAPMHWNDVFGDDLCINAVTSDAIDPVSQQPELKFCAVALSPVPAGR
VEPFLNDDDVSLADGAVAVAASAALANTISASAEDLDMPRIDALSSLLQLPPTPAPSLTD
LERVYLAGFVNGLRSAEAQGIDRVPVLPASAPFDATNRLYVDGLLAGLYSRAATTPAAAL
TTPHGATNAETSPASSGVRIVRARPKVTLLWASQTGNTESLTEGYATRLMESGFEIRTSC
MADCQASTLVKAQYVLLMTSTFGDGDPPDNAQSLWTQLNAEGAPRLDGVRFAVLALGDRN
YDQFCGHGRRLDERLAGHGALRLMERVDCDSEYQESADAWLESVIVRIKEEDAALYAVPP
GGMINAVVPGTVATKTRPAASRLVSNLRLNKQGAAKDTRYFSLSTGDSGLEYEAGDALGV
WPSNCPELVDELIALSGVSADAAVNVAGVGEMRLADALGKHYEIARPSPDLLAFVAARSD
NGALRDLLSPERKADLKQWLWGQQIADVLHEFPVNLTAAELTGMLKRLQPRLYSIASSPK
AHAGEVHLTVAAVRYSNGRRHRKGVSSTFLADRAGDVNVPVFVQKSAHFRPPHGSDTPMI
MVGPGTGVAPFRGFLHERRARGDRGRNWLFFGEQHAASDFYYRDELESMRDTGVLTRLDV
AFSRDQADKVYVQDRMREQGAQLWSWLEDGAHFYVCGDANRMAKDVDAALKEVVARHGGM
SDEKALEYVNRLAQEKRYARDVY