Protein Info for BPHYT_RS09705 in Burkholderia phytofirmans PsJN

Annotation: mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01479: mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase
  • amino acids 27 to 516 (490 residues), 641 bits, E=5.8e-197
• PF00483: NTP_transferase
  • amino acids 28 to 334 (307 residues), 164.7 bits, E=6e-52
• PF12804: NTP_transf_3
  • amino acids 29 to 161 (133 residues), 28.4 bits, E=3.6e-10
• PF01050: MannoseP_isomer
  • amino acids 362 to 512 (151 residues), 243.7 bits, E=1.4e-76
• PF07883: Cupin_2
  • amino acids 429 to 496 (68 residues), 47.3 bits, E=2.7e-16

Best Hits

KEGG orthology group: K00971, mannose-1-phosphate guanylyltransferase [EC: 2.7.7.22] K01809, mannose-6-phosphate isomerase [EC: 5.3.1.8] (inferred from 100% identity to bpy:Bphyt_1954)

Predicted SEED Role

"Mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22) ; Mannose-6-phosphate isomerase (EC 5.3.1.8)" (EC 2.7.7.22, EC 5.3.1.8)

MetaCyc Pathways

• colanic acid building blocks biosynthesis (11/11 steps found)
• GDP-mannose biosynthesis (4/4 steps found)
• β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation (2/3 steps found)
• D-mannose degradation I (1/2 steps found)
• D-mannose degradation II (1/2 steps found)
• mannitol degradation II (2/4 steps found)
• superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis (9/14 steps found)
• mannitol biosynthesis (1/3 steps found)
• 1,5-anhydrofructose degradation (2/5 steps found)
• β-(1,4)-mannan degradation (2/7 steps found)

KEGG Metabolic Maps

• Fructose and mannose metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.22, 5.3.1.8

Use Curated BLAST to search for 2.7.7.22 or 5.3.1.8

Sequence Analysis Tools

See B2T451 at UniProt or InterPro

Protein Sequence (517 amino acids)

>BPHYT_RS09705 mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase (Burkholderia phytofirmans PsJN)
MTAPVTATPADTRSTQVKSLNVKLKVHPVILAGGSGTRLWPMSREQHPKQLIGLLGDDSL
LQSTTRRLEGLEAGYPLTEQLVVVANEEQRFTTAEQLRTSGKPTRLILEPAGRDTAPALT
VAALSVAAQDEDGIMVVMPADHAVTDIDGFHAAVAAGVQHAAAGHIVTMGIVPTRAETGY
GYIRIGAALGTTNHTQEADAEVTGKVGAHKLDRFVEKPHLELAQRYIDSKEYWWNSGIFI
MRASTWLKAIRHFQPAIYEACDAAFAGGKADGDFFRLQRDAFSASPSNSIDYAVMEQLGN
DQSAASGVVVPLQAGWSDVGSWDAIWDISDKDADQNVGRGRVMFEGAASTFAHSEGRLIA
CVGTQDLVVVETADAILVADKSRVQDVKKIVGRIRNDGGLEAANHRKVHRPWGNYDSVDT
GERFQVKRIVVKPGARLSLQMHHHRAEHWIVVRGTALVTRGEERFIVSENESAYIPLGVT
HRLENPGKMPLEMIEVQSGSYLGEDDIVRFDDTYGRQ