Protein Info for BPHYT_RS09515 in Burkholderia phytofirmans PsJN

Annotation: phosphoribosylformylglycinamidine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1361 TIGR01735: phosphoribosylformylglycinamidine synthase" amino acids 7 to 1360 (1354 residues), 1872.1 bits, see alignment E=0 PF18076: FGAR-AT_N" amino acids 38 to 162 (125 residues), 133.1 bits, see alignment E=9.4e-43 PF18072: FGAR-AT_linker" amino acids 183 to 232 (50 residues), 87 bits, see alignment (E = 1.9e-28) PF02769: AIRS_C" amino acids 466 to 623 (158 residues), 111.6 bits, see alignment E=8.3e-36 amino acids 873 to 1032 (160 residues), 66.5 bits, see alignment E=6.3e-22 PF13507: GATase_5" amino acids 1105 to 1360 (256 residues), 343.9 bits, see alignment E=9.5e-107

Best Hits

Predicted SEED Role

"Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3) / Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3)" in subsystem De Novo Purine Biosynthesis (EC 6.3.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.3

Use Curated BLAST to search for 6.3.5.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T414 at UniProt or InterPro

Protein Sequence (1361 amino acids)

>BPHYT_RS09515 phosphoribosylformylglycinamidine synthase (Burkholderia phytofirmans PsJN)
MAHFSCFPGASALSDFRQTRLLETLTRIDPNITGVRGQYLHFVNAQTPLSAEDNAKIEAL
MHYGDPLEETKERDTAETFLVVPRFGTVSPWASKATDIAHLCGLTQVRRIERGVEYTVTL
KSGLLGGKKALSDEARAAVAAALHDRMTESVSSSRDHALHLFDELPAKPLQTVDILSNGR
GALETANTELGLALADDEIDYLVDAFTKLGRNPTDVELMMFAQANSEHCRHKIFNADWTI
DGEKQDISLFNMIRNTEKLNPRGTIVAYSDNSAIMAGGMAERWFPRTPADLGPSELPEHY
RRSNELTHTLMKVETHNHPTAISPFPGAATGAGGEIRDEGATGRGARPKAGLAGFTVSNL
ELPDGVEAWENARDAAQPLAQRNPDDKHEAYGRPDRIASPLQIMIDGPLGGAAFNNEFGR
PNLGGYFRAYEQNVAGLVRGYHKPIMIAGGIGNISDQHTHKHDLPEGSLLIQIGGPGMRI
GMGGGAASSMATGTNTAELDFDSVQRGNPEIERRAQEVINACWQLGEKNPILSIHDVGAG
GLSNAFPEVVDGAGKGARFDLRKIQLEESGLSPREIWSNEAQERYVLAIAPADLPAFAAM
CERERCPFAVIGTATAERQLKLIDSELNDDAAHQPVDMPMEVLLGKAPRMHRDVKRVERK
LEPVDVTGLALSDVATSVLRHPTVASKSFLITIGDRSVGGTTARDQMVGPWQVPVADVAI
TTMDYAGFTGEAMTMAERTPLAVIDAPASGRMAVGEAVTNIAAAPIASLDKLKLSANWMA
ACGAAGEDAALYDTVKAIGMELCPALGISIPVGKDSLSMRTKWEDRGVAKEVVAPVSLII
SAFAPVQDVRRHLTPQLRRTSDVGESVLIAIDLGRGKHRLGGSILAQVTQQVGDTVPDVD
DPEDLKRFFAAIQALNQDGKLLAYHDRSDGGLWATVCEMAFAGHVGVSLNVDMLVLDPNH
ESDYGDAKDWAKQTSGRREDRTIRALFNEELGAVVQVRASERDAVLAALREHGLSACSHV
IGKINERDTIEIYRDAKKIYDAPRTELHRTWSEVSWRIARLRDNPACADAEYDALSDAAD
PGISPILTFDPAEDVAAPFVGKSARPRVAILREQGVNSHLETAYAFDRAGFDAHDVHMSD
LLAGRANLADFAGAVACGGFSYGDTLGAGEGWAKAIRFNAQLADMFAAFFGREDTFALGI
CNGCQMMSSLASMIPGAEAWPKFTRNKSEKFEARFSLVEVQASPSLFFSGMEGSRIPVAI
AHGEGYADFSQQGDASKVAVAMRYVDHRGQATEQYPFNPNGSPNGITSVTTPDGRFTVLM
PHTERVHRAVQMSWHPEGWGEGATDASPWLRVFQNARRWLG