Protein Info for BPHYT_RS09490 in Burkholderia phytofirmans PsJN

Annotation: arylesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00657: Lipase_GDSL
  • amino acids 51 to 216 (166 residues), 67.9 bits, E=1.5e-22
• PF13472: Lipase_GDSL_2
  • amino acids 53 to 211 (159 residues), 97.6 bits, E=1.2e-31

Best Hits

Swiss-Prot: 40% identical to TESA_ECOL6: Thioesterase 1/protease 1/lysophospholipase L1 (tesA) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K10804, acyl-CoA thioesterase I [EC: 3.1.1.5 3.1.2.-] (inferred from 100% identity to bpy:Bphyt_1912)

MetaCyc: 40% identical to multifunctional acyl-CoA thioesterase I and protease I and lysophospholipase L1 (Escherichia coli K-12 substr. MG1655)
Acyl-CoA hydrolase. [EC: 3.1.2.20]; Oleoyl-[acyl-carrier-protein] hydrolase. [EC: 3.1.2.20, 3.1.2.14]; 3.1.2.14 [EC: 3.1.2.20, 3.1.2.14]; Lysophospholipase. [EC: 3.1.2.20, 3.1.2.14, 3.1.1.5]

Predicted SEED Role

"Arylesterase precursor (EC 3.1.1.2)" (EC 3.1.1.2)

MetaCyc Pathways

• superpathway of fatty acids biosynthesis (E. coli) (50/53 steps found)
• palmitate biosynthesis III (28/29 steps found)
• superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
• palmitate biosynthesis II (type II fatty acid synthase) (29/31 steps found)
• anteiso-branched-chain fatty acid biosynthesis (30/34 steps found)
• even iso-branched-chain fatty acid biosynthesis (30/34 steps found)
• odd iso-branched-chain fatty acid biosynthesis (30/34 steps found)
• tetradecanoate biosynthesis (mitochondria) (23/25 steps found)
• superpathway of unsaturated fatty acids biosynthesis (E. coli) (19/20 steps found)
• palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) (9/9 steps found)
• cis-vaccenate biosynthesis (5/5 steps found)
• stearate biosynthesis II (bacteria and plants) (5/6 steps found)
• palmitoleate biosynthesis IV (fungi and animals) (2/2 steps found)
• acyl-CoA hydrolysis (1/1 steps found)
• stearate biosynthesis IV (4/6 steps found)
• palmitoleate biosynthesis II (plants and bacteria) (1/2 steps found)
• phospholipid remodeling (phosphatidylethanolamine, yeast) (2/4 steps found)
• oleate biosynthesis I (plants) (1/3 steps found)
• bacterial bioluminescence (4/8 steps found)
• petroselinate biosynthesis (2/6 steps found)
• firefly bioluminescence (3/14 steps found)
• jasmonic acid biosynthesis (5/19 steps found)
• superpathway of mycolate biosynthesis (26/239 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Biosynthesis of plant hormones
• Biosynthesis of unsaturated fatty acids
• Bisphenol A degradation
• Fatty acid biosynthesis
• Glycerophospholipid metabolism
• Limonene and pinene degradation
• Ubiquinone and menaquinone biosynthesis
• alpha-Linolenic acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.5, 3.1.2.-, 3.1.2.20

Use Curated BLAST to search for 3.1.1.2 or 3.1.1.5 or 3.1.2.- or 3.1.2.14 or 3.1.2.20

Sequence Analysis Tools

See B2T409 at UniProt or InterPro

Protein Sequence (234 amino acids)

>BPHYT_RS09490 arylesterase (Burkholderia phytofirmans PsJN)
MVKRRLKVRAMTTRATALTTACGCAVVAASLFSVSSTARAANAPEPARPVIVVLGDSISA
EYGLPRDTGWVALMRQRLADEQIDYSVANASISGDTTSGGRARMPALMQRLKPSIVIVEL
GANDALRGVPLSTTEDNLRTIIEQAQQGHAKVVLVGMYVPPNYGPDYTQKFHGLYGELSK
QLRVPLVPFLLAGIADKPDMFQADQIHPTQQAQPVLLNNVWPAVKPLLRTSSPH