Protein Info for BPHYT_RS09385 in Burkholderia phytofirmans PsJN

Annotation: FAD-dependent oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 378 PF01266: DAO" amino acids 10 to 348 (339 residues), 204.6 bits, see alignment E=6.7e-64 PF13450: NAD_binding_8" amino acids 13 to 41 (29 residues), 23.3 bits, see alignment (E = 1.3e-08)

Best Hits

Swiss-Prot: 49% identical to OOXB_RHIME: Opine oxidase subunit B (ooxB) from Rhizobium meliloti (strain 1021)

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_1893)

MetaCyc: 43% identical to nopaline oxidase subunit B (Agrobacterium tumefaciens)
1.5.-.-

Predicted SEED Role

"Opine oxidase subunit B"

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T3Z0 at UniProt or InterPro

Protein Sequence (378 amino acids)

>BPHYT_RS09385 FAD-dependent oxidoreductase (Burkholderia phytofirmans PsJN)
MTLTSTDSPDVLVLGGGLVGSAVAYGLAREGARVTVLDEDDGGLRASRGNFGLVWIQGKG
YGLSPYARWSRSSATRWPGLAQALHNETGVDVALRQPGGFHFCFNDSELAEREKRLSTLQ
AELGDYPYQMLDAAEVRARIPQIGPAVIGASYSPMDGHVNPLKLLRGLHAAMQARGVRLV
SGERAERIEPQAQGFVVHGKRGTYRAARVVLAAGLGNRALAPFVGLDAPVAPNRGQVLVS
ERVARFLDYPTLNVRQTDEGSVQFGDSMEEVGFNDFTTTHVLADIARRGVRAFPMLQHVR
LVRMWAALRVYSPDGFPIYDHSEAHPGAFVVTCHSGVTLAAAHALRVAPWIMGAPMPDEL
PHFSARRFEHAGELTPAH