Protein Info for BPHYT_RS09295 in Burkholderia phytofirmans PsJN

Annotation: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01128: IspD
  • amino acids 6 to 232 (227 residues), 202 bits, E=1.1e-63
• TIGR00453: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
  • amino acids 7 to 231 (225 residues), 220.6 bits, E=8.4e-70
• PF12804: NTP_transf_3
  • amino acids 7 to 131 (125 residues), 41.4 bits, E=1.7e-14

Best Hits

Swiss-Prot: 100% identical to ISPD_PARPJ: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) from Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)

KEGG orthology group: K00991, 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase [EC: 2.7.7.60] (inferred from 100% identity to bpy:Bphyt_1875)

Predicted SEED Role

"2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (EC 2.7.7.60)" in subsystem Isoprenoid Biosynthesis or Teichoic and lipoteichoic acids biosynthesis or polyprenyl synthesis (EC 2.7.7.60)

MetaCyc Pathways

• methylerythritol phosphate pathway II (8/9 steps found)
• superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP) (10/12 steps found)
• taxadiene biosynthesis (engineered) (10/13 steps found)
• methylerythritol phosphate pathway I (7/9 steps found)
• isoprene biosynthesis I (7/10 steps found)
• superpathway of ergosterol biosynthesis II (11/26 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Terpenoid biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.60

Sequence Analysis Tools

See B2T3X2 at UniProt or InterPro

Protein Sequence (238 amino acids)

>BPHYT_RS09295 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (Burkholderia phytofirmans PsJN)
MTSRLFALIPCAGTGSRSGAAMPKQYRTVAGRDMLHYSLAAFDACSEFAQTLVVIAPDDA
HFDGRRFGGLRFAVRRSGGASRQASVLNGLHALAEFGARDDDWVLVHDAARPGITPALIR
TLIGALKDDPVGGIMALPVADTLKRIDATSSDGRIARTEARDGLWQAQTPQMFRIGMLRE
AILRAQADGHDLTDEASAIEWLGHAPKLVQGSLRNFKVTYPEDFDLAEAILSRPAAAS