Protein Info for BPHYT_RS09290 in Burkholderia phytofirmans PsJN

Annotation: helicase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1160 PF17757: UvrB_inter" amino acids 139 to 227 (89 residues), 77.1 bits, see alignment 3.2e-25 TIGR00580: transcription-repair coupling factor" amino acids 157 to 1083 (927 residues), 1048.1 bits, see alignment E=0 PF21132: MFD_D3" amino acids 379 to 453 (75 residues), 78.4 bits, see alignment 1.1e-25 PF02559: CarD_TRCF_RID" amino acids 482 to 538 (57 residues), 72.8 bits, see alignment 6.7e-24 PF04851: ResIII" amino acids 607 to 763 (157 residues), 37.3 bits, see alignment E=9.2e-13 PF00270: DEAD" amino acids 607 to 767 (161 residues), 88.2 bits, see alignment E=1.8e-28 PF00271: Helicase_C" amino acids 808 to 911 (104 residues), 68 bits, see alignment E=2.9e-22 PF03461: TRCF" amino acids 1011 to 1103 (93 residues), 90.8 bits, see alignment E=2e-29

Best Hits

KEGG orthology group: K03723, transcription-repair coupling factor (superfamily II helicase) [EC: 3.6.4.-] (inferred from 88% identity to bch:Bcen2424_1944)

Predicted SEED Role

"Transcription-repair coupling factor" in subsystem DNA-replication or Transcription factors bacterial

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.-

Use Curated BLAST to search for 3.6.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T3X1 at UniProt or InterPro

Protein Sequence (1160 amino acids)

>BPHYT_RS09290 helicase (Burkholderia phytofirmans PsJN)
MPDIAASSQYSPPVALVKAGQRFAFDGTHGSSDALLIARYHLAYREKVPLLAVVCESAVD
AQRLAQEIGFFAPEARVRLLPDWETLPYDTFSPHQDLVSERLATLHDLGEGRCDILLVPA
TTALYRMPPASFLAAYTFSFAQGERLDEARIKAQLTLAGYEHVSQVVRPGEYCVRGSLLD
LYPMGSPLPYRIDLFDDQVDSIRAFDPDTQRSLYPVKDVRLLPGREFPFDEAARTAFRSR
WRETFEGDPSRAAIYKDIGNGVPSAGIEYYLPLFFEDTATLFHYLPEGAQLAFVGDLDAA
IRRFTNDTKQRYNFLSHDRDRPILEPQRLFLSDEDFFTLAKPFARLSLPANAGGGWSTPL
PNLAIDRHAEDPVAGLRAYLDTTPNRVLFAAESAGRRETLLQLLADNHLRPVSSDSFQDW
LTSDERFSLGVAPLANGFAVPVDGIAIITETELYGPLARRAGRRRQEQASNVDSMVRDLS
ELKVGDPVVHSQHGIGRYMGLVTMDLGEGETEFLHLEYAGDSKLYVPVAQLHVISRYSGA
DPESAPLHSLGSGQWEKAKRKAAQQIRDTAAELLNLYARRALRQGHAFALEPKDYVKFAE
SFGFEETPDQAAAIAAVIGDMTSGKPMDRLVCGDVGFGKTEVALRAAFIAVMGGKQVALL
SPTTLLAEQHTQTFSDRFSDWPVRIAELSRFKSTKEVSAAIQQINEGTVDIVIGTHKLLS
SDVQFKRLGLVIIDEEHRFGVRQKEALKALRAEVDVLTLTATPIPRTLGMALEGLRDFSV
IATAPQKRLAIKTFVRREEDSVIREAMLRELKRGGQVYFLHNEVETIENRRQMLEALVPE
ARIAVAHGQMHERELERVMRDFVAQRANVLLCTTIIETGIDVPSANTILIHRADKFGLAQ
LHQLRGRVGRSHHQAYSYLLVHDPQGLTKQAQRRLEAIQQMEELGSGFYLAMHDLEIRGT
GEVLGDKQSGEIHEIGFQLYTDMLNDAVKALKEGKEPDLTAPLAATTEINLHASAILPAD
YCGDVQERLSLYKRLANCEHNDSIDGIQEELIDRFGKLPPQAHALVETHRLRLAAKPLGI
SKIDAGEAVIGLQFIPNPPIDAMRIIEMVQKHKHIKLAGQDKLRIETRSPDLAVRVATVK
ETLRALGTPSRGTATSAAAR