Protein Info for BPHYT_RS08815 in Burkholderia phytofirmans PsJN

Updated annotation (from data): L-asparaginase (EC 3.5.1.1)
Rationale: Specifically important for utilizing L-Asparagine. Automated validation from mutant phenotype: the predicted function (ASPARAGHYD-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: L-asparaginase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 49% identical to ASPG_WOLSU: L-asparaginase (ansA) from Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W)

KEGG orthology group: K01424, L-asparaginase [EC: 3.5.1.1] (inferred from 100% identity to bpy:Bphyt_1778)

MetaCyc: 48% identical to L-asparaginase 2 (Escherichia coli K-12 substr. MG1655)
Asparaginase. [EC: 3.5.1.1, 3.5.1.38, 3.5.5.4]

Predicted SEED Role

"L-asparaginase (EC 3.5.1.1)" in subsystem Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 3.5.1.1)

MetaCyc Pathways

L-glutamate and L-glutamine biosynthesis (7/7 steps found)
L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
ammonia assimilation cycle III (3/3 steps found)
L-glutamate biosynthesis I (2/2 steps found)
L-asparagine degradation I (1/1 steps found)
L-glutamine degradation I (1/1 steps found)
L-citrulline biosynthesis (6/8 steps found)
glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
L-asparagine degradation III (mammalian) (2/3 steps found)
superpathway of L-citrulline metabolism (8/12 steps found)
superpathway of L-aspartate and L-asparagine biosynthesis (2/4 steps found)
N-(1-deoxy-D-fructos-1-yl)-L-asparagine degradation (2/5 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.1, 3.5.1.38

Use Curated BLAST to search for 3.5.1.1 or 3.5.1.38 or 3.5.5.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T3M5 at UniProt or InterPro

Protein Sequence (347 amino acids)

>BPHYT_RS08815 L-asparaginase (EC 3.5.1.1) (Burkholderia phytofirmans PsJN)
MNTSTSSSAMPSDEGAMPLLPRIVVLATGGTIAGAAASATNTSGYQAGVIGVEQLLAVVP
ALSTVARMEREQIASVDSKDMAMPLWTTLAQRINTLLADDEIDGVVVTHGTDTLEETAYL
LHLTIKSDKPVVLTAAMRPASALSADGPLNLLNAVTVAAQASARGQGVLVAFNNRIHSAR
DVVKTSTYAVDAFHSPEIGALGWVQDGRVEFQRGVVRPHTLATEFVIGAQWPHVEIVLSY
AGVSRIAVDALVAAGVRGIVVAGTGNGSIHASVQQALADAASQGVAVVRASRVGSGHVMR
NGAAADDALGFVSAGSLNPYKARVLLMLALAAGATGPMALQKIFDTY