Protein Info for BPHYT_RS08700 in Burkholderia phytofirmans PsJN

Annotation: tRNA-dihydrouridine synthase A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 333 TIGR00742: tRNA dihydrouridine synthase A" amino acids 10 to 324 (315 residues), 455.7 bits, see alignment E=4.8e-141 PF01207: Dus" amino acids 14 to 311 (298 residues), 274 bits, see alignment E=7.6e-86

Best Hits

Swiss-Prot: 61% identical to DUSA_PSEPK: tRNA-dihydrouridine(20/20a) synthase (dusA) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K05539, tRNA-dihydrouridine synthase A [EC: 1.-.-.-] (inferred from 100% identity to bpy:Bphyt_1755)

MetaCyc: 60% identical to tRNA-dihydrouridine synthase A (Escherichia coli K-12 substr. MG1655)
1.1.1.-

Predicted SEED Role

"tRNA dihydrouridine synthase A"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-

Use Curated BLAST to search for 1.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T3K2 at UniProt or InterPro

Protein Sequence (333 amino acids)

>BPHYT_RS08700 tRNA-dihydrouridine synthase A (Burkholderia phytofirmans PsJN)
MSSPHTPSPRRVSVAPMMDWTDRHCRSLHRVISRHTWLYTEMVTTGALLHGDVPRHLAFT
PEEAPVALQLGGSEPDDLARSAKLGEQWGYDEINLNCGCPSERVQRGAFGACLMNEPQLV
ADCVKAMRDAVSVPVTVKHRIGVDAVEEYGFVRDFVGTIAEAGCNVFIVHARNAILKGLS
PKENREIPPLKYEYAYQLKRDFPQLEIIINGGIKTLDEVEAHLEHVDGVMLGREAYHNPY
VLADVDARFYGAAQTPLTREQVEAKLIEYCAAEMVRGTYLGAITRHALGLYRGEAGARGW
RRVLSDSKRLAARDLTIFDEARQHLREPIEIFE