Protein Info for BPHYT_RS08575 in Burkholderia phytofirmans PsJN

Annotation: leucyl/phenylalanyl-tRNA--protein transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 245 PF03588: Leu_Phe_trans" amino acids 36 to 210 (175 residues), 244.3 bits, see alignment E=2.5e-77 TIGR00667: leucyl/phenylalanyl-tRNA--protein transferase" amino acids 40 to 220 (181 residues), 189 bits, see alignment E=3.1e-60

Best Hits

Swiss-Prot: 100% identical to LFTR_PARPJ: Leucyl/phenylalanyl-tRNA--protein transferase (aat) from Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)

KEGG orthology group: K00684, leucyl/phenylalanyl-tRNA--protein transferase [EC: 2.3.2.6] (inferred from 100% identity to bpy:Bphyt_1732)

Predicted SEED Role

"Leucyl/phenylalanyl-tRNA--protein transferase (EC 2.3.2.6)" in subsystem Protein degradation (EC 2.3.2.6)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.2.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T3H9 at UniProt or InterPro

Protein Sequence (245 amino acids)

>BPHYT_RS08575 leucyl/phenylalanyl-tRNA--protein transferase (Burkholderia phytofirmans PsJN)
MVPWLGPDDPFPPVERALGATSGAPGLLAASGDLLPSRLIDAYRRGIFPWYSDGQPVLWW
SPDPRMVLRPAEFKVAPSLRKTLRRVLREDAWEIRVDHDFASVMRACAQAPRRGQRGTWI
TADVVEAYSSLHRVGDAHSIETWFEGKRVGGLYGVSFGKMFFGESMFAEVTDASKMALAA
LVGHLRRHEIEMIDCQQNTSHLASLGGREITRKSFIAHVRASVEAPPIPWRFDKTALLEV
VAPAS