Protein Info for BPHYT_RS08410 in Burkholderia phytofirmans PsJN

Annotation: dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 313 PF00248: Aldo_ket_red" amino acids 32 to 290 (259 residues), 171 bits, see alignment E=1.6e-54

Best Hits

Swiss-Prot: 41% identical to AK1A1_BOVIN: Aldo-keto reductase family 1 member A1 (AKR1A1) from Bos taurus

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_1700)

MetaCyc: 43% identical to L-arabinose reductase LarA (Aspergillus niger)
RXN-8773 [EC: 1.1.1.431]; Aldehyde reductase. [EC: 1.1.1.431, 1.1.1.21]

Predicted SEED Role

"2,5-diketo-D-gluconic acid reductase (EC 1.1.1.-)" (EC 1.1.1.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.-

Use Curated BLAST to search for 1.1.1.- or 1.1.1.21 or 1.1.1.431

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T3E7 at UniProt or InterPro

Protein Sequence (313 amino acids)

>BPHYT_RS08410 dehydrogenase (Burkholderia phytofirmans PsJN)
MIESSDFRSRKIALDHGGGRVPALGFGTLIPDAATTTSATRDALEAGYRHFDCAERYRNE
REVGDALRAGLAAGGIAREEIFVTTKLWNSNHRPERVEPAFDASLERLGLDYLDLYLIHT
PFAFKPGDEQDPRDENGDVIYDKGVTLLDTWSAMEELVDRGRCRAIGLSDIGLDELRPLY
ESARIKPAVVQVEAHPYLPETELLEFCKEKGIVFLAFAPLGHGIRPGPLEDPVILQIAER
IGKTPAQVLLAWAIQRGGALLTTPRTAARARENFDIAALPAEAFDEINRIETRQRYNEVV
KTGSPGFIPRRPQ