Protein Info for BPHYT_RS08330 in Burkholderia phytofirmans PsJN

Annotation: histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1833 PF07714: PK_Tyr_Ser-Thr" amino acids 92 to 249 (158 residues), 59.7 bits, see alignment E=1.9e-19 PF14531: Kinase-like" amino acids 100 to 247 (148 residues), 28.3 bits, see alignment E=6.9e-10 PF00069: Pkinase" amino acids 102 to 252 (151 residues), 92 bits, see alignment E=2.9e-29 PF13191: AAA_16" amino acids 292 to 481 (190 residues), 156.1 bits, see alignment E=8.3e-49 PF13185: GAF_2" amino acids 1307 to 1450 (144 residues), 40.2 bits, see alignment E=2.7e-13 PF01590: GAF" amino acids 1308 to 1449 (142 residues), 42.8 bits, see alignment 5.1e-14 PF13492: GAF_3" amino acids 1308 to 1451 (144 residues), 29.8 bits, see alignment E=4.8e-10 TIGR00229: PAS domain S-box protein" amino acids 1459 to 1581 (123 residues), 55 bits, see alignment 4.5e-19 PF00989: PAS" amino acids 1464 to 1570 (107 residues), 33.9 bits, see alignment 1.8e-11 PF13426: PAS_9" amino acids 1476 to 1574 (99 residues), 41.8 bits, see alignment 7.4e-14 PF00512: HisKA" amino acids 1606 to 1673 (68 residues), 35.6 bits, see alignment (E = 5e-12) PF02518: HATPase_c" amino acids 1717 to 1827 (111 residues), 84.1 bits, see alignment 6.1e-27

Best Hits

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_1683)

Predicted SEED Role

"Signal transduction histidine kinase CheA (EC 2.7.3.-)" in subsystem Bacterial Chemotaxis or Flagellar motility or Two-component regulatory systems in Campylobacter (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.3.-

Use Curated BLAST to search for 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T3D2 at UniProt or InterPro

Protein Sequence (1833 amino acids)

>BPHYT_RS08330 histidine kinase (Burkholderia phytofirmans PsJN)
MDLTGYELEPLHDDGDFSLYRARRPRHAVSVLALVATRPASHSLTRLEHEYGLAPLLDSS
WAAQPLALYRRREPPMLVLDDDGGEPLYRSLGRPLELTRWLRIAVNLARVIGHVHRCGLV
HKDIKPANVLVDGNGNVRLTGFGIASQLPHEHQPPAPPEIVAGTFAYMAPEQTGRMNRSI
DTRSDLYSLGVTLYEMLTGALPFTASDAMEWIHCHIARRPTPPGERVDGLPKPVEGIVLK
LLAKAAEDRYQTAAGVEADLRSCLAAWEAHHRIQAFPLGAHDASDRLLVPEKLYGREAQI
EALVSAFDRVVGSGAVELVLISGYPGIGKSSVVNELHRVLVPPRGLFASGKFDQYKRDIP
YVPFAQAFQTLVRNLLSQSDAEVEPWRHALLEALGPNAQLMVNLIPELALIIGEQPPAPV
LPPQDAQNRFQIVFRRFLGVFARPEHPLALFIDDLQWVDTATLDLLAHLVTHPEVKHVLL
VGAYRDNEVDASHPFARTLESISHAEGKVQRIELAPLTTENVTQLVADSLHCDGASAGPL
AQLVHEKTGGNPFFAIQFLTALADEDLLTFDQRAAGWCWDLWRIRSKGFTENVADLMAAK
LSRLPAPTRDALGQLACLGNIAEVIALTWVQGGSEETMHANLWEAVRTGLVFRVANAYEF
AHDRVQEAAYALIPADERSAAHLRIGRALVSRTPSDALEDAIFDIVNHLNRGAALIVTEP
EREQAVALNLIAGRRAMNSTAYAAARSYLAQGAALLSPDAWAQRYDNTFDLYLAFSECEY
LVGDFAKADALVDMMLARARSNLDRAKIFSLRIELYQLAGRYDESFAVALAALRDFGILF
PENDQDIQAALDHALRDVHVNQAGRRVDELVDVPVATDPAIRAIVNLLLQAMNCAFAARP
AFYPLITLKAVNLSLQHGNTENSSFAYGNYALMLVSLIGDIPTAVQVSEMSLKLNEKFGN
RRLNGKLLHLYGAHVNFWRCHIANNLPVLERATAACLEAGDLVFAGNVAFNAVWQTIEKG
AALEDVQALAEKYAALARESHNDAVYELIRVERQFVISLRGKTTEPLNLNDETFDETACF
EAIVQSNFGCAIGVYRIIKVVLAFLDGRYSEALEAADRAEAVLSSVMALAIEPTYHFFHA
LILTALYPQVSGEQQQACKHILAAKLRKLEMWAEHCPENYQNRYALVCAELARVEGRDLD
AMRLYEQAVHSAHENGFPHHEGIANELAGRFYLSRSLRTSADAYLRNARHCFALWGADGK
VAQLDAEFPRLANQDGGNATSTLSFDWRQFDAATLLKASQALSSEIELDGLIERLMTIAL
QTSGADRGLLVVPQQPDGYRIEAEARINGQGIALNREPLAHFAVPAGILRYVIHTQKSVI
IDDALTPNMFSQDSYVASGATRSLFCLPLVRQGTLSGLLYLENTATSHVFTARRSSLLDL
LASQAAISLENTRLYADLQEREAKVRRLVDSNIIGIHIWDFEGGVVEANDAFLRIVGYSR
DDLVSARIRWPDLTPPEWRELDERVRVELTTTGSAKPFEKEYIRKDGSRVPVLVGAAAFG
DREGQGVAFILDLTDQKRAQQHVRDSERRYVGLQMELAHSNRVATMGQLSASIAHEVKQP
ITATVAYASAALRWLAARPPNLDEVREALNRIVADGGRANSIVDRTRAFFKKEPQRKDGL
DINETILELIAFMRSEASKHGVELSAKLADGLPQIHGDRVQLQQVMLNLMINALEAMSVK
EAGERTLVIRSGRSGIDELCISVEDSGPGLDTEHPEGVFEAFFTTKPNGLGMGLPICRSI
VESHGGRLWVTSNSAGGATFQFTLPAQGDVAHT