Protein Info for BPHYT_RS08050 in Burkholderia phytofirmans PsJN

Annotation: pseudouridine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 626 PF01479: S4" amino acids 214 to 256 (43 residues), 30.7 bits, see alignment 2.1e-11 PF00849: PseudoU_synth_2" amino acids 275 to 408 (134 residues), 63.9 bits, see alignment E=2e-21 TIGR00093: pseudouridine synthase" amino acids 279 to 439 (161 residues), 167.5 bits, see alignment E=1e-53

Best Hits

KEGG orthology group: K06178, ribosomal large subunit pseudouridine synthase B [EC: 5.4.99.12] (inferred from 100% identity to bpy:Bphyt_1629)

Predicted SEED Role

"Ribosomal large subunit pseudouridine synthase B (EC 4.2.1.70)" in subsystem Two cell division clusters relating to chromosome partitioning (EC 4.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T378 at UniProt or InterPro

Protein Sequence (626 amino acids)

>BPHYT_RS08050 pseudouridine synthase (Burkholderia phytofirmans PsJN)
MTHTHDTDSSESERAVPSARADETRTTQASAGEGERPAQTTEADGEDRPRRGLRRGPRSL
IARRRAGAKTKGAEGAPAQGAPVVTEAPPDGEVVAQVRMPRKDAGAKGQGPRRNAAGAKR
EGAPRDGAPREGQREGQRERQPREGGQRQNNRRGGEAPAAAAVAGQDDLFSYVTSPAFDA
DNSATGGVRAPMLRRGKPAAPKRVLAADDDAPKLHKVLAEAGMGSRRDMEELIVAGRVSV
NGEPAHIGQRIMPTDQVRINGKPVKRKLANKPPRVLLYHKPTGEIVSHADPEGRASVFDN
LPPMKTAKWLAVGRLDFNTEGLLMLTTSGDLANRFMHPRYSVEREYAVRVVGELAEGMRQ
KLLNGVELEDGPANFLRIRDGGGEGTNHWYHVALAEGRNREVRRMFEAAGLMVSRLIRTR
HGPISLPKGLKRGRWEELEDNQVRALMASVGLKAPTEEKGGRREAPERKQPDPMQTSMGF
IGREPVLMSHSRFDQQQPRGQGQGRRGAAGGGFGGGAGAGFGGGYGNRGAGRGAGGFSGP
MGGGAGGPRGGREVDGNRAPSGNGNRAAGGGKRAGGSGGPNPGSGGGNRGPGGNRGGAGN
RAGGGNANAGGANRGGGAPRGRSRGR