Protein Info for BPHYT_RS07875 in Burkholderia phytofirmans PsJN

Annotation: CoA transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01144: CoA_trans
  • amino acids 23 to 262 (240 residues), 100.2 bits, E=1.2e-32
• PF16957: Mal_decarbox_Al
  • amino acids 192 to 271 (80 residues), 27.3 bits, E=1.2e-10

Best Hits

KEGG orthology group: None (inferred from 100% identity to bpy:Bphyt_1595)

Predicted SEED Role

"Acetyl-CoA:acetoacetyl-CoA transferase, alpha subunit (EC 2.8.3.8)" in subsystem Acetyl-CoA fermentation to Butyrate (EC 2.8.3.8)

MetaCyc Pathways

• acetyl-CoA fermentation to butanoate (6/7 steps found)
• acetoacetate degradation (to acetyl CoA) (2/2 steps found)
• pyruvate fermentation to acetone (3/5 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (8/13 steps found)
• 4-aminobutanoate degradation V (3/7 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (5/10 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (10/17 steps found)
• gallate degradation III (anaerobic) (5/11 steps found)
• L-glutamate degradation XI (reductive Stickland reaction) (2/7 steps found)
• succinate fermentation to butanoate (2/7 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Butanoate metabolism
• Propanoate metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.8.3.8

Use Curated BLAST to search for 2.8.3.8

Sequence Analysis Tools

See B2T344 at UniProt or InterPro

Protein Sequence (663 amino acids)

>BPHYT_RS07875 CoA transferase (Burkholderia phytofirmans PsJN)
MSAGFQHPFLATANNIGRGKIVSAQQAVRLIRPGNTVATGGFVGIGFAEEIAVALEQLFL
DSAPDDSDAPRNLTLVYAGGQGDGARKGLNHFAHQGLVRRVIGGHWGLVPGLQRLAVEGH
IEAYNLPQGVISQLFRDIAAHRPGHLSTVGLDTFVDPRNGGGKLNARTTEDLVSLISVNG
ADCLLYKTFPVDVAIVRGTTADANGNVTMEKEALTLESLAIAMAARNSGGIVIVQVERIA
EAGTLNPRQVKIPGILVDCVVVAKQENHWQTFAESYSPGFASELRIATSSIPGMALTERK
VIARRASFELMANSVVNLGIGMPEGIASVANEEKVCDLFTMTTEPGVIGGIPAGGLNFGA
ATNTDAIIDQPYQFDFYDGGGLDIAFLGLAQVDREGNLNVSKFGPKLAGAGGFINISQAA
KKVVFVGTFNAGKLDVNIKDGRLQILQDGVCQKFVEAVEHRTFGGRYASERQQPVLYITE
RCVFTLTPDGLELVEIAPGVDLQRDILSQMGFAPVIKSPPRLMDARIFDNKPMGLRAMLL
RLTLSERFSYDADKNMLFINFEGHKVMTVDDVEAVRAEVERVLSGLDVRPHAIVNYDNFS
IAPEVIDAYSEMVNGLVARYYSDVTRYTTSSFLRMKLGDALQRRSVATYIYESPEEASSH
EAR