Protein Info for BPHYT_RS07615 in Burkholderia phytofirmans PsJN

Annotation: glycogen synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 486 TIGR02095: glycogen/starch synthase, ADP-glucose type" amino acids 4 to 485 (482 residues), 568.4 bits, see alignment E=5.8e-175 PF08323: Glyco_transf_5" amino acids 6 to 245 (240 residues), 266.7 bits, see alignment E=4.3e-83 PF00534: Glycos_transf_1" amino acids 292 to 461 (170 residues), 53.7 bits, see alignment E=3.8e-18 PF13692: Glyco_trans_1_4" amino acids 304 to 447 (144 residues), 44.5 bits, see alignment E=3.8e-15

Best Hits

Swiss-Prot: 57% identical to GLGA_FRATN: Glycogen synthase (glgA) from Francisella tularensis subsp. novicida (strain U112)

KEGG orthology group: K00703, starch synthase [EC: 2.4.1.21] (inferred from 100% identity to bpy:Bphyt_1542)

MetaCyc: 56% identical to glycogen synthase (Escherichia coli K-12 substr. MG1655)
Starch synthase. [EC: 2.4.1.21]

Predicted SEED Role

"Glycogen synthase, ADP-glucose transglucosylase (EC 2.4.1.21)" in subsystem Glycogen metabolism (EC 2.4.1.21)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.21

Use Curated BLAST to search for 2.4.1.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T2Z4 at UniProt or InterPro

Protein Sequence (486 amino acids)

>BPHYT_RS07615 glycogen synthase (Burkholderia phytofirmans PsJN)
MTIRALHVASELYPLLKTGGLADVAGALPPALIERGADVRVLLPGFPAVVAGLTDLTPVA
QLGRRFDAPGVTLERGTLASNGLVVYVIRAATLYDRPGNPYLNDEHVPYGDNAQRFALLG
WVAAQLAQQLDPAWSPQIIHAHDWHAGLAPAYLKAAERQHGKRFARSVFTVHNLAYQGVF
PAHQFGQLGLPDDFFSVHGVEFYGQMSFLKAGLYYSDRITTVSPTYAREIQTLAQGGGLD
GLLRHRAHDLSGILNGVDYTVWCPGTDTLLENHYTATRLAGKLACKEALQKRFGLAQKSD
ALLFGVVSRLTEQKGLDLLLEAVPEIIKHGGQLVVFGTGDPALENGLERVAHTHPESVAV
ELGFDETLAHTIVAGSDVIAVPSRFEPCGLTQLYALAYGSLPLVHCVGGLADTVVDASLE
NLADDLATGFVFERFEPKGIGAAIRRAFALYGRRTEWKATQRRAMRQDFGWGASAERYLA
LYRELA