Protein Info for BPHYT_RS07555 in Burkholderia phytofirmans PsJN
Updated annotation (from data): Histidine ammonia-lyase (EC 4.3.1.3)
Rationale: Specifically important for utilizing L-Histidine. Automated validation from mutant phenotype: the predicted function (HISTIDINE-AMMONIA-LYASE-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: histidine ammonia-lyase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to HUTH_PARPJ: Histidine ammonia-lyase (hutH) from Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)
KEGG orthology group: K01745, histidine ammonia-lyase [EC: 4.3.1.3] (inferred from 100% identity to bpy:Bphyt_1530)MetaCyc: 64% identical to histidase subunit (Pseudomonas putida)
Histidine ammonia-lyase. [EC: 4.3.1.3]
Predicted SEED Role
"Histidine ammonia-lyase (EC 4.3.1.3)" in subsystem Histidine Degradation (EC 4.3.1.3)
MetaCyc Pathways
- L-histidine degradation II (5/5 steps found)
- L-histidine degradation VI (7/8 steps found)
- L-histidine degradation I (4/4 steps found)
- L-histidine degradation III (4/6 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 4.3.1.3
Use Curated BLAST to search for 4.3.1.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See B2T2Y2 at UniProt or InterPro
Protein Sequence (506 amino acids)
>BPHYT_RS07555 Histidine ammonia-lyase (EC 4.3.1.3) (Burkholderia phytofirmans PsJN) MTLKPGYLTLPQLRQIAREHVALQLDPASHAAIDACAQAVADIAAKGEPAYGINTGFGRL ASTHIPHDQLELLQRNLVLSHAVGVGEPMSRPVVRLLIALKLSSLGRGHSGIRREVMEAL ITLYNADVLPVIPVKGSVGASGDLAPLAHMSATLLGVGEVFAKGERMPATEGLALVGLKP LTLQAKEGLALLNGTQASTALALYNMFAIEDLYRTALVSGALSVDAAMGSVKPFDARIHE LRGHQGQIDAAGAYRSLLQGSGINVSHADCDKVQDPYSLRCQPQVMGACLDQMRHAANVL LLEANAVSDNPLIFPDTGEVLSGGNFHAEPVAFAADNLALAAAEIGALAERRIALLIDAT LSGLPPFLVRDGGVNSGFMIAHVTAAALASENKTLAHPASVDSLPTSANQEDHVSMATFA ARKLGDIAENTANILSIELLAAAQGVDLRAPHKTSPSLQKVMDAVRKDVAHYELDHYFAP DIAAVTRLVQDGTIAKLSPLSFASEQ