Protein Info for BPHYT_RS07335 in Burkholderia phytofirmans PsJN

Annotation: nitrate ABC transporter permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 355 signal peptide" amino acids 1 to 39 (39 residues), see Phobius details PF09084: NMT1" amino acids 67 to 282 (216 residues), 128.9 bits, see alignment E=1.3e-41

Best Hits

KEGG orthology group: K02051, sulfonate/nitrate/taurine transport system substrate-binding protein (inferred from 100% identity to bpy:Bphyt_1486)

Predicted SEED Role

"ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components" in subsystem Alkanesulfonate assimilation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T2T9 at UniProt or InterPro

Protein Sequence (355 amino acids)

>BPHYT_RS07335 nitrate ABC transporter permease (Burkholderia phytofirmans PsJN)
MLQRIRRAVARRVSELTRRTALSAAALAAATLFTGVHAAAQTTDKPAARVADKVRVNLAW
LPQGSTGGILLAHAKGYYREAGLDVSVMRGYGGQRTVNEVDGGLFEFGYGDPVSVMLNRA
HGGHAVMVGAINTRWPGAMCYLERPGFKVSSLKDLAGMTLGGGGASPVQNIMPAWLKQNG
MAPDAIKTVRLDPAVINTALLQKRIDLSECWEGASLPVQAALVKRAGQQLGKVYYRDFGL
DMMGNGIVTTDSFVAQHPDVVKRFVAATYRGYALMREHPQEAADAIAAQYPVLDKTILLQ
QIVETNGLITDDPAQHKTGWLRPARMDATAAFVSQAFGTGAKVKAADLYTNRFVE