Protein Info for BPHYT_RS07320 in Burkholderia phytofirmans PsJN

Annotation: dihydrodipicolinate synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00701: DHDPS
  • amino acids 2 to 285 (284 residues), 130.8 bits, E=2.1e-42

Best Hits

KEGG orthology group: K01714, dihydrodipicolinate synthase [EC: 4.2.1.52] (inferred from 100% identity to bpy:Bphyt_1483)

Predicted SEED Role

"4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7) / N-acetylneuraminate lyase (EC 4.1.3.3)" in subsystem Sialic Acid Metabolism (EC 4.1.3.3, EC 4.3.3.7)

MetaCyc Pathways

• aspartate superpathway (23/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
• L-lysine biosynthesis I (9/9 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• superpathway of N-acetylneuraminate degradation (17/22 steps found)
• L-lysine biosynthesis III (6/7 steps found)
• L-lysine biosynthesis VI (6/7 steps found)
• L-lysine biosynthesis II (7/9 steps found)
• dipicolinate biosynthesis (3/4 steps found)
• N-acetylneuraminate and N-acetylmannosamine degradation II (1/3 steps found)
• superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation (3/6 steps found)
• N-acetylneuraminate and N-acetylmannosamine degradation I (1/4 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Lysine biosynthesis
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.52, 4.3.3.7

Use Curated BLAST to search for 4.1.3.3 or 4.2.1.52 or 4.3.3.7

Sequence Analysis Tools

See B2T2T6 at UniProt or InterPro

Protein Sequence (302 amino acids)

>BPHYT_RS07320 dihydrodipicolinate synthetase (Burkholderia phytofirmans PsJN)
MFAPVITPFTKDLRIDAPRFVAFCRWLVGQGAGLALFGTNSEANSLGLTERHALLDDVIK
AGVDPAHLLPGTGSCALPDAIALTRHACEAGCAGALMLPPFFYKGVSDDGLFAYYSDVIQ
AIGSDQLRVLLYHIPQFTGVPITHTLIERLITAYPRTVVGIKDSSGDWANTEAMLRKFPG
FAVFPASEALLGKALPLGAAGCISATANIQPQAIASLLNATTLEERAVWEGKVATVRLAV
QAQPMIPALKHIVAHFANDESWTRVRPPLTPMNPAQSGALLQQLQALDFSMPAMATLATV
AA