Protein Info for BPHYT_RS06850 in Burkholderia phytofirmans PsJN

Annotation: DNA polymerase III subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1193 PF02811: PHP" amino acids 23 to 170 (148 residues), 44.2 bits, see alignment E=7e-15 TIGR00594: DNA polymerase III, alpha subunit" amino acids 132 to 864 (733 residues), 456.3 bits, see alignment E=1.3e-140 PF07733: DNA_pol3_alpha" amino acids 353 to 608 (256 residues), 290.2 bits, see alignment E=4.2e-90 PF17657: DNA_pol3_finger" amino acids 611 to 777 (167 residues), 178.2 bits, see alignment E=2.2e-56 PF14579: HHH_6" amino acids 851 to 999 (149 residues), 70.3 bits, see alignment E=3.3e-23 PF01336: tRNA_anti-codon" amino acids 1080 to 1140 (61 residues), 31.4 bits, see alignment 3.7e-11

Best Hits

Predicted SEED Role

"DNA polymerase III alpha subunit (EC 2.7.7.7)" in subsystem DNA-replication (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T2I8 at UniProt or InterPro

Protein Sequence (1193 amino acids)

>BPHYT_RS06850 DNA polymerase III subunit alpha (Burkholderia phytofirmans PsJN)
MPPTQPSLLVQQPLATQLPAYAELHCLTNFSFLRGASHPHELVEQAMSRGYSALAITDEC
SLAGVVRAHTALKEIKTERERQQKEREDKANTAQSEAPSEDGTQIEASQQETAPQETAEA
EAPKPLPHLIIGSELNLTDETGDPFCTLVALATNRNGYGNLSELITLARSRADKGSYRLG
PSDFTASLPHLAHLKTLPDCVLMLVPQRTATLSHTLRCAHWLASFAAQRAWIALELWQTG
SDDLQIDALRMISKASGLPLVAAGGVLMHARSRKPLQDTMTAIGLVTPLSQCGHALEANA
ERHMRTRVRLGKLYPRDTLEETLRIAALCRFSLDELKYEYPEELVPAGESPSSYLRKLVM
AGAMERWPKGIDLKRIGQIEKELGLIADLKYEKYFLTVHDIVSFARSRNILCQGRGSAAN
SIVCYCLHVTEIDPVHMNMLIERFISRARNEPPDIDVDFEHQRREEVIQYIYTKYGRHRA
ALTASLITYHARSALKDVGKALGLEVSLIERISKSQQWWDGTDAVAKYLAEAGFDANSHI
TQNLIRLTKELRAFPRHLSQHVGGFVIAKDKLSRLVPIENATMKDRSVIEWDKDDIDALK
LLKVDVLALGMLSAIRRALEFVALRRGFPKFSVQDIPREDRAVYEMCGHADTIGVFQIES
RAQQSMLPRLKPNKYYDLVIEVAIVRPGPIQGGMVHPYLRRKQGLEAVDYAKDELRPVLE
RTLGVPIFQEQVMHLAMVAAKYTGEQADQLRRAMAAWRRSGNLAKYQKDLNERMLKRGYE
QEFIDRICKQIEGFGEYGFPESHAASFALLVYLSAWLKRYEPAAFLAGLLNSQPLGFYSP
SQLVQDAKRHGVEVLPPDVTLSDWESTFERRGHEGQRISADETCLHRQQAVLSAQQLRDL
SLRRLTVSRTVRRAAQRLTARVFQPSHTYGARGPAVRIGMHLIKGFSQAAAERIMAARNE
APFTDVDDLARRAALTRRDLEALAAANALVSIAGHRREAWWAVTAQHTVPKLLRDAPIAE
APLMLPQAAESREIVDDYASLGLTLNRHPLALLRARLAQHRFRTAAELAACQHGTLARAC
GIVTVRQRPGTANGTVFVSIEDETGSINVIVWPSLVEKQRKVLLGASLLAVYGVLQRDDG
VATGGDQAGNGVSANQAKGKRKNKGEVIHLVAHRLEDHSEWLGELATASRDFH