Protein Info for BPHYT_RS06750 in Burkholderia phytofirmans PsJN
Annotation: fructose-2,6-bisphosphatase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K01834, phosphoglycerate mutase [EC: 5.4.2.1] (inferred from 100% identity to bpy:Bphyt_1369)Predicted SEED Role
"Alpha-ribazole-5'-phosphate phosphatase (EC 3.1.3.73)" in subsystem Cobalamin synthesis or Coenzyme B12 biosynthesis (EC 3.1.3.73)
MetaCyc Pathways
- adenosylcobalamin biosynthesis II (aerobic) (30/33 steps found)
- adenosylcobalamin biosynthesis I (anaerobic) (30/36 steps found)
- superpathway of adenosylcobalamin salvage from cobinamide I (8/8 steps found)
- superpathway of adenosylcobalamin salvage from cobinamide II (8/9 steps found)
- 2-methyladeninyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP (3/3 steps found)
- 5-hydroxybenzimidazolyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP (3/3 steps found)
- 5-methoxy-6-methylbenzimidazolyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP (3/3 steps found)
- 5-methoxybenzimidazolyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP (3/3 steps found)
- 5-methylbenzimidazolyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP (3/3 steps found)
- adeninyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP (3/3 steps found)
- adenosylcobalamin biosynthesis from adenosylcobinamide-GDP I (3/3 steps found)
- benzimidazolyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP (3/3 steps found)
- 4-methylphenyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP (2/3 steps found)
- phenyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP (2/3 steps found)
- adenosylcobalamin biosynthesis from adenosylcobinamide-GDP II (2/4 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Glycolysis / Gluconeogenesis
- Porphyrin and chlorophyll metabolism
Isozymes
Compare fitness of predicted isozymes for: 3.1.3.73, 5.4.2.1
Use Curated BLAST to search for 3.1.3.73 or 5.4.2.1
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See B2T2H3 at UniProt or InterPro
Protein Sequence (238 amino acids)
>BPHYT_RS06750 fructose-2,6-bisphosphatase (Burkholderia phytofirmans PsJN) MPDYQLPKRRRIFLMRHGDVTYFDDSGRAIDPETVPLNANGREQASAAGRVFAEQQVRFD RVIVSGLPRTIETARRVLAEINQQIELEIEPAWEEIRGGKLSNIPLQDIEAAFLGVFDGI VPESTRFLDGETIGALFDRVLPAVAALREDSSWDTVLLVLHGGVNRAILSHALTAGGRTF FGHLAQATGCINALDVGAAPRDWVLRTINYSPPSPLHRDVRNTTMEMLYAQFTQYKRS