Protein Info for BPHYT_RS06415 in Burkholderia phytofirmans PsJN

Annotation: uracil phosphoribosyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01091: uracil phosphoribosyltransferase
  • amino acids 12 to 215 (204 residues), 249.6 bits, E=1.2e-78
• PF14681: UPRTase
  • amino acids 13 to 214 (202 residues), 248.4 bits, E=2.4e-78

Best Hits

Swiss-Prot: 100% identical to UPP_PARPJ: Uracil phosphoribosyltransferase (upp) from Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)

KEGG orthology group: K00761, uracil phosphoribosyltransferase [EC: 2.4.2.9] (inferred from 97% identity to bug:BC1001_1041)

Predicted SEED Role

"Uracil phosphoribosyltransferase (EC 2.4.2.9)" in subsystem De Novo Pyrimidine Synthesis or LMPTP YwlE cluster (EC 2.4.2.9)

MetaCyc Pathways

• superpathway of pyrimidine nucleobases salvage (4/4 steps found)
• pyrimidine nucleobases salvage II (2/2 steps found)
• pyrimidine nucleobases salvage I (1/1 steps found)
• superpathway of pyrimidine ribonucleosides salvage (7/10 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.9

Use Curated BLAST to search for 2.4.2.9

Sequence Analysis Tools

See B2T2A6 at UniProt or InterPro

Protein Sequence (216 amino acids)

>BPHYT_RS06415 uracil phosphoribosyltransferase (Burkholderia phytofirmans PsJN)
MTQDSRFPNLFILDHPLIQHKLSHMRDRDTSTRTFRELLREITLLMGYEITRNLPMTTRR
LTTPLVEIDAPVIAGKKLAIVPVLRAGIGMSDGLLELVPSARVGHIGVYRAEDHRPVEYL
VRLPDLEDRVFILCDPMVATGYSAVHAVDVLKRRNVAGENIMFLALVAAPEGVQVFQDAH
PDVKLYVASLDSHLNEHAYIVPGLGDAGDRLFGTKN