Protein Info for BPHYT_RS06385 in Burkholderia phytofirmans PsJN

Annotation: ribosomal RNA large subunit methyltransferase H

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 156 PF02590: SPOUT_MTase" amino acids 1 to 154 (154 residues), 170.8 bits, see alignment E=1.1e-54 TIGR00246: rRNA large subunit m3Psi methyltransferase RlmH" amino acids 2 to 155 (154 residues), 164 bits, see alignment E=1.3e-52

Best Hits

Swiss-Prot: 100% identical to RLMH_PARPJ: Ribosomal RNA large subunit methyltransferase H (rlmH) from Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)

KEGG orthology group: K00783, hypothetical protein (inferred from 100% identity to bpy:Bphyt_1296)

MetaCyc: 52% identical to 23S rRNA m3psi1915 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-11592 [EC: 2.1.1.177]

Predicted SEED Role

"LSU m3Psi1915 methyltransferase RlmH" in subsystem Ribosome biogenesis bacterial

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.177

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T2A0 at UniProt or InterPro

Protein Sequence (156 amino acids)

>BPHYT_RS06385 ribosomal RNA large subunit methyltransferase H (Burkholderia phytofirmans PsJN)
MKLHILAVGHKMPDWIATGFDEYTKRMPPELRIELREIKPEQRSSGRQADSVMAAERLKI
EAALPKNARIVALDERGKDWTTMQLAGALPGWQQDGRDVAFLIGGADGLDPQLKARSDML
LRVSSLTLPHAMVRVLLAEQLYRAWTITQNHPYHRV